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Apoptosis-related microRNA-145-5p enhances the effects of pheophorbide a-based photodynamic therapy in oral cancer

Authors
 Sook Moon  ;  Do Kyeong Kim  ;  Jin Kim 
Citation
 Oncotarget, Vol.8(21) : 35184-35192, 2017 
Journal Title
 Oncotarget 
Issue Date
2017
MeSH
Cell Line, Tumor ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Chlorophyll/analogs & derivatives* ; Chlorophyll/pharmacology ; Gene Expression Regulation, Neoplastic/drug effects ; Humans ; MicroRNAs/genetics* ; Mouth Neoplasms/drug therapy ; Mouth Neoplasms/genetics* ; Mouth Neoplasms/metabolism ; Oligonucleotide Array Sequence Analysis ; Photochemotherapy ; Photosensitizing Agents/pharmacology* ; Reactive Oxygen Species/metabolism
Keywords
microRNA ; oral cancer ; pheophorbide a ; photodynamic therapy ; phototoxicity
Abstract
MicroRNAs (miRNAs) regulate key biological processes, and their aberrant expression has been related to cancer development. Photodynamic therapy (PDT) has emerged as one of the most promising modalities for cancer treatment. However, the application of PDT has been limited to superficially localized human cancerous and precancerous lesions. To increase the usefulness of both PDT and miRNAs in cancer therapy, this study investigated whether apoptosis-related miRNA expression is influenced by PDT in oral cancer and whether miRNAs can enhance PDT efficacy. To achieve this goal, we performed a miRNA array-based comparison of apoptosis-related miRNA expression patterns following PDT using pheophorbide a (Pa) as a photosensitizer. After Pa-PDT, 13.1% of the miRNAs were down-regulated, and 16.7% of the miRNAs were up-regulated. Representative miRNAs were selected according to expression difference: miR-9-5p, miR-32-5p, miR-143-3p, miR-145-5p, miR-192-5p, miR-193a-5p, miR-204-5p, miR-212-3p, miR-338-3p, and miR-451a. Among them, only miR-145-5p showed the consistent reduction repeatedly in all cell lines after Pa-PDT. Further, the combined treatment of a miR-145-5p mimic and Pa-PDT increased phototoxicity, reactive oxygen species generation, and apoptotic cell death, suggesting that miRNAs expression could be a useful marker for enhancing the therapeutic effect of Pa-PDT. This study will provide a promising strategy for introducing miRNA as cancer therapy.
Files in This Item:
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DOI
10.18632/oncotarget.17059
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
Yonsei Authors
김진(Kim, Jin)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/154265
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