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Application of genetically engineered Salmonella typhimurium for interferon-gamma-induced therapy against melanoma

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dc.contributor.author박성하-
dc.contributor.author김병모-
dc.date.accessioned2017-11-02T08:11:10Z-
dc.date.available2017-11-02T08:11:10Z-
dc.date.issued2017-
dc.identifier.issn0959-8049-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/154174-
dc.description.abstractSalmonella have been experimentally used as anti-cancer agents, because they show selective growth in tumours. In this study, we genetically modified attenuated Salmonella typhimurium to express and secrete interferon-gamma (IFN-γ) as a tumouricidal agent to enhance the therapeutic efficacy of Salmonella. IFN-γ was fused to the N-terminal region (residues 1-160) of SipB (SipB160) for secretion from bacterial cells. Attenuated S. typhimurium expressing recombinant IFN-γ (S. typhimurium (IFN-γ)) invaded the melanoma cells and induced cytotoxicity. Subcutaneous administration of S. typhimurium (IFN-γ) also efficiently inhibited tumour growth and prolonged the survival of C57BL/6 mice bearing B16F10 melanoma compared with administration of phosphate-buffered saline (PBS), unmodified S. typhimurium or S. typhimurium expressing empty vector (S. typhimurium [Vec]) in a natural killer (NK) cell-dependent manner. Moreover, genetically modified Salmonella, including S. typhimurium (IFN-γ), showed little toxicity to normal tissues with no observable adverse effects. However, S. typhimurium (IFN-γ)-mediated tumour suppression was attributed to direct killing of tumour cells rather than to stable anti-tumour immunity. Collectively, these results suggest that tumour-targeted therapy using S. typhimurium (IFN-γ) has potential for melanoma treatment.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier Science Ltd-
dc.relation.isPartOfEUROPEAN JOURNAL OF CANCER-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBlotting, Western/methods-
dc.subject.MESHDisease Models, Animal-
dc.subject.MESHHumans-
dc.subject.MESHImmunity, Innate-
dc.subject.MESHImmunotherapy/methods*-
dc.subject.MESHInterferon-gamma/biosynthesis*-
dc.subject.MESHKiller Cells, Natural/immunology-
dc.subject.MESHMacrophages/metabolism-
dc.subject.MESHMelanoma, Experimental/therapy*-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHOrganisms, Genetically Modified/metabolism*-
dc.subject.MESHSalmonella typhimurium/metabolism*-
dc.subject.MESHSalmonella typhimurium/pathogenicity-
dc.subject.MESHSkin Neoplasms/therapy*-
dc.subject.MESHTumor Cells, Cultured-
dc.titleApplication of genetically engineered Salmonella typhimurium for interferon-gamma-induced therapy against melanoma-
dc.typeArticle-
dc.publisher.locationEngland-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Internal Medicine-
dc.contributor.googleauthorWonsuck Yoon-
dc.contributor.googleauthorYoo Chang Park-
dc.contributor.googleauthorJinseok Kim-
dc.contributor.googleauthorYang Seok Chae-
dc.contributor.googleauthorJung Hye Byeon-
dc.contributor.googleauthorSang-Hyun Min-
dc.contributor.googleauthorSungha Park-
dc.contributor.googleauthorYoung Yoo-
dc.contributor.googleauthorYong Keun Park-
dc.contributor.googleauthorByeong Mo Kim-
dc.identifier.doi10.1016/j.ejca.2016.10.010-
dc.contributor.localIdA01512-
dc.relation.journalcodeJ00809-
dc.identifier.eissn1879-0852-
dc.identifier.pmid27883926-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0959804916324947-
dc.subject.keywordGenetically modified Salmonella typhimurium (S. typhimurium)-
dc.subject.keywordInterferon-gamma (IFN-γ)-
dc.subject.keywordMelanoma-
dc.subject.keywordSipB160-
dc.subject.keywordTreatment option for melanoma-
dc.contributor.alternativeNamePark, Sung Ha-
dc.contributor.affiliatedAuthorPark, Sung Ha-
dc.citation.titleEuropean Journal of Cancer-
dc.citation.volume70-
dc.citation.startPage48-
dc.citation.endPage61-
dc.identifier.bibliographicCitationEUROPEAN JOURNAL OF CANCER, Vol.70 : 48-61, 2017-
dc.date.modified2017-11-01-
dc.identifier.rimsid42138-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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