Cited 4 times in
Identificaiton of Novel Immunogenic Human Papillomavirus Type 16 E7-Specific Epitopes Restricted to HLA-A*33;03 for Cervical Cancer Immunotherapy.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 공훈영 | - |
dc.contributor.author | 김성훈 | - |
dc.contributor.author | 임종백 | - |
dc.date.accessioned | 2017-11-01T08:31:16Z | - |
dc.date.available | 2017-11-01T08:31:16Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/153322 | - |
dc.description.abstract | Purpose : To identify new immunogenic HLA-A*33;03-restricted epitopes from the human papillomavirus (HPV) 16 E7 protein for immunotherapy against cervical cancer. Materials and Methods : We synthesized fourteen overlapping 15-amino acid peptides and measured intracellular interferon-γ (IFN-γ) production in PBMC and CD8+ cytotoxic T lymphocytes (CTLs) after sensitization with these peptides using flow cytometry and ELISpot assay. The immunogenicity of epitopes was verified using a 51Cr release assay with SNU1299 cells. Results : Among the fourteen 15-amino acid peptides, E749-63 (RAHYNIVTFCCKCDS) demonstrated the highest IFN-γ production from peripheral blood mononuclear cells (PBMCs), and CD8+ CTLs sensitized with E749-63 showed higher cytotoxic effect against SNU1299 cells than did CD8+ CTLs sensitized with other peptides or a negative control group. Thirteen 9- or 10-amino acid overlapping peptides spanning E749-63, E750-59 (AHYNIVTFCC), and E752-61 (YNIVTFCCKC) induced significantly higher IFN-γ production and cytotoxic effects against SNU1299 cells than the other peptides and negative controls, and the cytotoxicity of E750-59- and E752-61-sensitized PBMCs was induced via the cytolytic effect of CD8+ CTLs. Conclusion : We identified E750-59 and E752-61 as novel HPV 16 E7 epitopes for HLA-A*33;03. CD8+ CTL sensitized with these peptides result in an antitumor effect against cervical cancer cells. These epitopes could be useful for immune monitoring and immunotherapy for cervical cancer and HPV 16-related diseases including anal cancer and oropharyngeal cancer. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Yonsei University | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | CD8-Positive T-Lymphocytes/immunology | - |
dc.subject.MESH | CD8-Positive T-Lymphocytes/metabolism | - |
dc.subject.MESH | Epitopes/immunology* | - |
dc.subject.MESH | Epitopes/therapeutic use | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | HLA-A Antigens* | - |
dc.subject.MESH | Human papillomavirus 16/immunology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunotherapy* | - |
dc.subject.MESH | Interferon-gamma/analysis | - |
dc.subject.MESH | Interferon-gamma/biosynthesis* | - |
dc.subject.MESH | Leukocytes, Mononuclear/immunology | - |
dc.subject.MESH | Leukocytes, Mononuclear/metabolism | - |
dc.subject.MESH | T-Lymphocytes, Cytotoxic/immunology | - |
dc.subject.MESH | T-Lymphocytes, Cytotoxic/metabolism | - |
dc.subject.MESH | Uterine Cervical Neoplasms/therapy* | - |
dc.title | Identificaiton of Novel Immunogenic Human Papillomavirus Type 16 E7-Specific Epitopes Restricted to HLA-A*33;03 for Cervical Cancer Immunotherapy. | - |
dc.type | Article | - |
dc.publisher.location | Korea (South) | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Yonsei Biomedical Research Center | - |
dc.contributor.googleauthor | Sunghoon Kim | - |
dc.contributor.googleauthor | Hye Won Chung | - |
dc.contributor.googleauthor | Hoon Young Kong | - |
dc.contributor.googleauthor | Jong-Baeck Lim | - |
dc.identifier.doi | 10.3349/ymj.2017.58.1.43 | - |
dc.contributor.localId | A00595 | - |
dc.contributor.localId | A03403 | - |
dc.contributor.localId | A00164 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 27873494 | - |
dc.subject.keyword | HLA-A*33 | - |
dc.subject.keyword | 03 | - |
dc.subject.keyword | HPV 16 E7 | - |
dc.subject.keyword | cervical cancer | - |
dc.subject.keyword | epitopes | - |
dc.subject.keyword | immunotherapy | - |
dc.contributor.alternativeName | Kong, Hoon Young | - |
dc.contributor.alternativeName | Kim, Sung Hoon | - |
dc.contributor.alternativeName | Lim, Jong Baeck | - |
dc.contributor.affiliatedAuthor | Kim, Sung Hoon | - |
dc.contributor.affiliatedAuthor | Lim, Jong Baeck | - |
dc.contributor.affiliatedAuthor | Kong, Hoon Young | - |
dc.citation.title | Yonsei Medical Journal | - |
dc.citation.volume | 58 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 43 | - |
dc.citation.endPage | 50 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.58(1) : 43-50, 2017 | - |
dc.date.modified | 2017-11-01 | - |
dc.identifier.rimsid | 41569 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.