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The Effect of Ambient Titanium Dioxide Microparticle Exposure to the Ocular Surface on the Expression of Inflammatory Cytokines in the Eye and Cervical Lymph Nodes

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dc.contributor.author김현창-
dc.contributor.author이형근-
dc.date.accessioned2017-10-26T08:03:04Z-
dc.date.available2017-10-26T08:03:04Z-
dc.date.issued2016-
dc.identifier.issn0146-0404-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/152876-
dc.description.abstractPurpose: To investigate the ocular immune response following exposure to airborne titanium dioxide (TiO2) microparticles. Methods: Rats in the TiO2-exposed group (n = 10) were exposed to TiO2 particles for 2 hours twice daily for 5 days, while the controls (n = 10) were not. Corneal staining score and tear lactic dehydrogenase (LDH) activity were measured to evaluate ocular surface damage, serum immunoglobulin (Ig) G and E were assayed by using enzyme-linked immunosorbent assay, and the size of cervical lymph nodes was measured. In addition, the expression of interleukin (IL)-4, IL-17, and interferon (IFN)-γ in the anterior segment of the eyeball and cervical lymph nodes was measured by immunohistochemistry, real-time reverse transcription-polymerase chain reaction (RT-PCR), and Western blot analysis. Results: Median corneal staining score (3.0), tear LDH activity (0.24 optical density [OD]), and cervical lymph node size (36.9 mm2) were significantly higher in the TiO2-exposed group than in the control group (1.0, 0.13 OD, and 26.7 mm2, respectively). Serum IgG and IgE levels were found to be significantly elevated in the TiO2-exposed group (P = 0.021 and P = 0.021, respectively). Interleukin 4 expression was increased in the anterior segment of the eyeball and lymph nodes following TiO2 exposure, as measured by immunostaining, real-time RT-PCR, and Western blot. In addition, IL-17 and IFN-γ levels were also increased following TiO2 exposure compared to controls as measured by immunostaining. Conclusions: Exposure to airborne TiO2 induced ocular surface damage. The Type 2 helper T-cell pathway seems to play a dominant role in the ocular immune response following airborne TiO2 exposure.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherAssociation For Research In Vision And Ophthalmology (Arvo)-
dc.relation.isPartOfINVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleThe Effect of Ambient Titanium Dioxide Microparticle Exposure to the Ocular Surface on the Expression of Inflammatory Cytokines in the Eye and Cervical Lymph Nodes-
dc.typeArticle-
dc.publisher.locationUnited States-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Preventive Medicine-
dc.contributor.googleauthorYoungsub Eom-
dc.contributor.googleauthorJong Suk Song-
dc.contributor.googleauthorHyun Kyu Lee-
dc.contributor.googleauthorBoram Kang-
dc.contributor.googleauthorHyeon Chang Kim-
dc.contributor.googleauthorHyung Keun Lee-
dc.contributor.googleauthorHyo Myung Kim-
dc.identifier.doi10.1167/iovs.16-19944-
dc.contributor.localIdA03303-
dc.contributor.localIdA01142-
dc.relation.journalcodeJ01187-
dc.identifier.eissn1552-5783-
dc.identifier.pmid27918832-
dc.identifier.urlhttp://iovs.arvojournals.org/article.aspx?articleid=2591384-
dc.contributor.alternativeNameKim, Hyeon Chang-
dc.contributor.alternativeNameLee, Hyung Keun-
dc.contributor.affiliatedAuthorLee, Hyung Keun-
dc.contributor.affiliatedAuthorKim, Hyeon Chang-
dc.citation.volume57-
dc.citation.number15-
dc.citation.startPage6580-
dc.citation.endPage6590-
dc.identifier.bibliographicCitationINVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol.57(15) : 6580-6590, 2016-
dc.date.modified2017-10-24-
dc.identifier.rimsid40501-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Preventive Medicine (예방의학교실) > 1. Journal Papers

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