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The Effect of Ambient Titanium Dioxide Microparticle Exposure to the Ocular Surface on the Expression of Inflammatory Cytokines in the Eye and Cervical Lymph Nodes
DC Field | Value | Language |
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dc.contributor.author | 김현창 | - |
dc.contributor.author | 이형근 | - |
dc.date.accessioned | 2017-10-26T08:03:04Z | - |
dc.date.available | 2017-10-26T08:03:04Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0146-0404 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/152876 | - |
dc.description.abstract | Purpose: To investigate the ocular immune response following exposure to airborne titanium dioxide (TiO2) microparticles. Methods: Rats in the TiO2-exposed group (n = 10) were exposed to TiO2 particles for 2 hours twice daily for 5 days, while the controls (n = 10) were not. Corneal staining score and tear lactic dehydrogenase (LDH) activity were measured to evaluate ocular surface damage, serum immunoglobulin (Ig) G and E were assayed by using enzyme-linked immunosorbent assay, and the size of cervical lymph nodes was measured. In addition, the expression of interleukin (IL)-4, IL-17, and interferon (IFN)-γ in the anterior segment of the eyeball and cervical lymph nodes was measured by immunohistochemistry, real-time reverse transcription-polymerase chain reaction (RT-PCR), and Western blot analysis. Results: Median corneal staining score (3.0), tear LDH activity (0.24 optical density [OD]), and cervical lymph node size (36.9 mm2) were significantly higher in the TiO2-exposed group than in the control group (1.0, 0.13 OD, and 26.7 mm2, respectively). Serum IgG and IgE levels were found to be significantly elevated in the TiO2-exposed group (P = 0.021 and P = 0.021, respectively). Interleukin 4 expression was increased in the anterior segment of the eyeball and lymph nodes following TiO2 exposure, as measured by immunostaining, real-time RT-PCR, and Western blot. In addition, IL-17 and IFN-γ levels were also increased following TiO2 exposure compared to controls as measured by immunostaining. Conclusions: Exposure to airborne TiO2 induced ocular surface damage. The Type 2 helper T-cell pathway seems to play a dominant role in the ocular immune response following airborne TiO2 exposure. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Association For Research In Vision And Ophthalmology (Arvo) | - |
dc.relation.isPartOf | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | The Effect of Ambient Titanium Dioxide Microparticle Exposure to the Ocular Surface on the Expression of Inflammatory Cytokines in the Eye and Cervical Lymph Nodes | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Preventive Medicine | - |
dc.contributor.googleauthor | Youngsub Eom | - |
dc.contributor.googleauthor | Jong Suk Song | - |
dc.contributor.googleauthor | Hyun Kyu Lee | - |
dc.contributor.googleauthor | Boram Kang | - |
dc.contributor.googleauthor | Hyeon Chang Kim | - |
dc.contributor.googleauthor | Hyung Keun Lee | - |
dc.contributor.googleauthor | Hyo Myung Kim | - |
dc.identifier.doi | 10.1167/iovs.16-19944 | - |
dc.contributor.localId | A03303 | - |
dc.contributor.localId | A01142 | - |
dc.relation.journalcode | J01187 | - |
dc.identifier.eissn | 1552-5783 | - |
dc.identifier.pmid | 27918832 | - |
dc.identifier.url | http://iovs.arvojournals.org/article.aspx?articleid=2591384 | - |
dc.contributor.alternativeName | Kim, Hyeon Chang | - |
dc.contributor.alternativeName | Lee, Hyung Keun | - |
dc.contributor.affiliatedAuthor | Lee, Hyung Keun | - |
dc.contributor.affiliatedAuthor | Kim, Hyeon Chang | - |
dc.citation.volume | 57 | - |
dc.citation.number | 15 | - |
dc.citation.startPage | 6580 | - |
dc.citation.endPage | 6590 | - |
dc.identifier.bibliographicCitation | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol.57(15) : 6580-6590, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 40501 | - |
dc.type.rims | ART | - |
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