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Non-Thermal Atmospheric Pressure Plasma Efficiently Promotes the Proliferation of Adipose Tissue-Derived Stem Cells by Activating NO-Response Pathways

Authors
 Jeongyeon Park  ;  Hyunyoung Lee  ;  Hae June Lee  ;  Gyoo Cheon Kim  ;  Do Young Kim  ;  Sungbum Han  ;  Kiwon Song 
Citation
 SCIENTIFIC REPORTS, Vol.6 : 39298, 2016 
Journal Title
SCIENTIFIC REPORTS
Issue Date
2016
MeSH
Adipose Tissue/cytology ; Cell Proliferation/drug effects* ; Humans ; Nitric Oxide/metabolism* ; Plasma Gases/metabolism* ; Signal Transduction ; Stem Cells/drug effects* ; Stem Cells/physiology*
Abstract
Non-thermal atmospheric pressure plasma (NTAPP) is defined as a partially ionized gas with electrically charged particles at atmospheric pressure. Our study showed that exposure to NTAPP generated in a helium-based dielectric barrier discharge (DBD) device increased the proliferation of adipose tissue-derived stem cells (ASCs) by 1.57-fold on an average, compared with untreated cells at 72?h after initial NTAPP exposure. NTAPP-exposed ASCs maintained their stemness, capability to differentiate into adipocytes but did not show cellular senescence. Therefore, we suggested that NTAPP can be used to increase the proliferation of ASCs without affecting their stem cell properties. When ASCs were exposed to NTAPP in the presence of a nitric oxide (NO) scavenger, the proliferation-enhancing effect of NTAPP was not obvious. Meanwhile, the proliferation of NTAPP-exposed ASCs was not much changed in the presence of scavengers for reactive oxygen species (ROS). Also, Akt, ERK1/2, and NF-κB were activated in ASCs after NTAPP exposure. These results demonstrated that NO rather than ROS is responsible for the enhanced proliferation of ASCs following NTAPP exposure. Taken together, this study suggests that NTAPP would be an efficient tool for use in the medical application of ASCs both in vitro and in vivo.
Files in This Item:
T201605312.pdf Download
DOI
10.1038/srep39298
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Do Young(김도영) ORCID logo https://orcid.org/0000-0002-0194-9854
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/152768
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