Cited 28 times in
Inhibition of microRNA-449a prevents IL-1β-induced cartilage destruction via SIRT1
DC Field | Value | Language |
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dc.contributor.author | 김성환 | - |
dc.contributor.author | 박광환 | - |
dc.contributor.author | 이경미 | - |
dc.contributor.author | 이진우 | - |
dc.contributor.author | 최우진 | - |
dc.contributor.author | 윤동석 | - |
dc.date.accessioned | 2017-10-26T07:55:04Z | - |
dc.date.available | 2017-10-26T07:55:04Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1063-4584 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/152690 | - |
dc.description.abstract | OBJECTIVE: SIRT1 has anti-inflammatory as well as protective effects in chondrocytes. The object of this study was to investigate whether microRNA-449a regulates expression of SIRT1, which inhibits expression of catabolic genes in IL-1β-induced cartilage destruction. MATERIALS AND METHODS: MicroRNA-449a expression was determined in OA chondrocytes and IL-1β-induced chondrocytes by real-time PCR. MicroRNA-449a binding sites on the 3'-UTR of SIRT1 mRNA and binding site conservation were examined using microRNA target prediction tools. SIRT1-overexpressing or knockdown chondrocytes were transfected with microRNA-449a or anti-microRNA-449a mimic and stimulated by IL-1β. Expression of catabolic and anabolic genes was examined by real-time PCR and western blotting. Finally, positive effects of anti-microRNA-449a on expression of these genes were confirmed by western analysis of OA chondrocytes. RESULTS: Expression of microRNA-449a was increased in OA chondrocytes and IL-1β-induced chondrocytes. MMP-13 expression was enhanced, whereas type II collagen and SIRT1 expression were decreased in IL-1β-induced chondrocytes. SIRT1 overexpression resulted in decreased expression of catabolic genes such as MMPs and ADAMTSs in response to IL-1β, but these effects were moderated by microRNA-449a. Suppression of microRNA-449a by anti-microRNA-449a inhibited expression of catabolic genes despite IL-1β stimulation, but these effects were abolished in SIRT1 knockdown chondrocytes. Furthermore, expression of catabolic genes was decreased and expression of type II collagen as well as SIRT1 was restored by anti-microRNA-449a in OA chondrocytes as well as in IL-1β-induced chondrocytes. CONCLUSION: Silencing of microRNA-449a had a protective effect, inhibiting catabolic gene expression and restoring anabolic gene expression, by targeting SIRT1 in IL-1β-induced cartilage destruction. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | W.B. Saunders | - |
dc.relation.isPartOf | OSTEOARTHRITIS AND CARTILAGE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Inhibition of microRNA-449a prevents IL-1β-induced cartilage destruction via SIRT1 | - |
dc.type | Article | - |
dc.publisher.location | England | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Orthopedic Surgery | - |
dc.contributor.googleauthor | K.W. Park | - |
dc.contributor.googleauthor | K.-M. Lee | - |
dc.contributor.googleauthor | D.S. Yoon | - |
dc.contributor.googleauthor | K.H. Park | - |
dc.contributor.googleauthor | W.J. Choi | - |
dc.contributor.googleauthor | J.W. Lee | - |
dc.contributor.googleauthor | S.-H. Kim | - |
dc.identifier.doi | 10.1016/j.joca.2016.07.002 | - |
dc.contributor.localId | A01437 | - |
dc.contributor.localId | A04619 | - |
dc.contributor.localId | A03230 | - |
dc.contributor.localId | A04121 | - |
dc.contributor.localId | A00592 | - |
dc.relation.journalcode | J03039 | - |
dc.identifier.eissn | 1522-9653 | - |
dc.relation.journalsince | 1993 | - |
dc.identifier.pmid | 27421775 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S1063458416301765?via%3Dihub | - |
dc.contributor.alternativeName | Kim, Sung Hwan | - |
dc.contributor.alternativeName | Park, Kwang Hwan | - |
dc.contributor.alternativeName | Lee, Kyoung Mi | - |
dc.contributor.alternativeName | Lee, Jin Woo | - |
dc.contributor.alternativeName | Choi, Woo Jin | - |
dc.contributor.affiliatedAuthor | Park, Kwang Hwan | - |
dc.contributor.affiliatedAuthor | Lee, Kyoung Mi | - |
dc.contributor.affiliatedAuthor | Lee, Jin Woo | - |
dc.contributor.affiliatedAuthor | Choi, Woo Jin | - |
dc.contributor.affiliatedAuthor | Kim, Sung Hwan | - |
dc.citation.volume | 24 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 2153 | - |
dc.citation.endPage | 2161 | - |
dc.identifier.bibliographicCitation | OSTEOARTHRITIS AND CARTILAGE, Vol.24(12) : 2153-2161, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 39699 | - |
dc.type.rims | ART | - |
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