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Proteomic Analysis and Identification of Paracrine Factors in Mesenchymal Stem Cell-Conditioned Media under Hypoxia

Authors
 Suk-Won Song  ;  Kyung-Eun Kim  ;  Jung-Won Choi  ;  Chang Youn Lee  ;  Jiyun Lee  ;  Hyang-Hee Seo  ;  Kyu Hee Lim  ;  Soyeon Lim  ;  Seahyong Lee  ;  Sang Woo Kim  ;  Ki-Chul Hwang 
Citation
 CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, Vol.40(1~2) : 400-410, 2016 
Journal Title
 CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 
ISSN
 1015-8987 
Issue Date
2016
Abstract
BACKGROUND/AIMS: We previously showed that a hypoxic environment modulates the antiarrhythmic potential of mesenchymal stem cells. METHODS: To investigate the mechanism by which secreted proteins contribute to the pathogenesis of antiarrhythmic potential in mesenchymal stem cells, we used two-dimensional electrophoresis combined with MALDI-TOF-MS to perform a proteomic analysis to compare the paracrine media produced by normoxic and hypoxic cells. RESULTS: The proteomic analysis revealed that 66 protein spots out of a total of 231 matched spots indicated differential expression between the normoxic and hypoxic conditioned media of mesenchymal stem cells. Interestingly, two tropomyosin isoforms were dramatically increased in the hypoxic conditioned medium of mesenchymal stem cells. An increase in tropomyosin was confirmed using Western blot to analyze the conditioned media between normoxic and hypoxic cells. In a network analysis based on gene ontology (GO) Molecular Function by GeneMANIA analysis, most of the identified proteins were found to be involved in the regulation of heart processes. CONCLUSION: Our results show that hypoxia up-regulates tropomyosin and other secreted proteins which suggests that tropomyosin may be involved in regulating proarrhythmic and antiarrhythmic functions.
Files in This Item:
T201604920.pdf Download
DOI
10.1159/000452555
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
Yonsei Authors
Song, Suk Won(송석원) ORCID logo https://orcid.org/0000-0002-9850-9707
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/152675
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