Cited 16 times in
Cigarette Smoke Extract Causes Injury in Primary Retinal Ganglion Cells via Apoptosis and Autophagy
DC Field | Value | Language |
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dc.contributor.author | 김찬윤 | - |
dc.contributor.author | 성공제 | - |
dc.contributor.author | 이광현 | - |
dc.contributor.author | 홍사민 | - |
dc.date.accessioned | 2017-10-26T07:52:53Z | - |
dc.date.available | 2017-10-26T07:52:53Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0271-3683 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/152636 | - |
dc.description.abstract | AIMS: To investigate whether tobacco smoke directly injures retinal ganglion cells (RGCs) and to evaluate the mechanisms of cell death. METHODS: Primary rat RGCs were harvested from 3- or 4-day-old newborn rats and exposed to cigarette smoke extract (CSE). Cell viability was determined by adenosine 5'-triphosphate (ATP) assay. Apoptosis was evaluated by terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick-end labeling (TUNEL) and real-time reverse transcription polymerase chain reaction (RT-PCR) for Bcl-2 family. Autophagy was also assessed by Western immunoblots for light chain (LC) 3B. RESULTS: When the primary RGCs were exposed to CSE for 2 h, cell viability decreased in a dose-dependent manner, as measured by ATP assay. In the presence of 0.05% CSE, the RGC viability was 77.68% ± 7.60% compared to the control cells; in the presence of 1.0% CSE, viability was 47.48% ± 2.56% of the control cells. As determined by TUNEL, CSE increased the apoptotic RGCs in a dose-dependent manner. In the presence of 0.05% CSE, the apoptosis was 26.55% ± 1.97% of the control cells; in the presence of 2.5% CSE, it was 41.07% ± 3.75% of the control cells. When apoptosis was evaluated using real-time RT-PCR, exposure to 0.05% CSE resulted in significantly increased expression of Bad, Bax, Bcl-2, and Bcl-XL mRNA. When autophagy was assessed by Western immunoblots, exposure to 0.05% CSE significantly increased the expression of LC3B II. CONCLUSIONS: Our data suggest that CSE directly injures primary RGCs, and both cell death mechanisms of apoptosis and autophagy seem to be related to this CSE-induced RGC damage. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Informa Healthcare | - |
dc.relation.isPartOf | CURRENT EYE RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Cigarette Smoke Extract Causes Injury in Primary Retinal Ganglion Cells via Apoptosis and Autophagy | - |
dc.type | Article | - |
dc.publisher.location | England | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Ophthalmology | - |
dc.contributor.googleauthor | Kwanghyun Lee | - |
dc.contributor.googleauthor | Samin Hong | - |
dc.contributor.googleauthor | Gong Je Seong | - |
dc.contributor.googleauthor | Chan Yun Kim | - |
dc.identifier.doi | 10.3109/02713683.2015.1119856 | - |
dc.contributor.localId | A01946 | - |
dc.contributor.localId | A04994 | - |
dc.contributor.localId | A04395 | - |
dc.contributor.localId | A01035 | - |
dc.relation.journalcode | J00665 | - |
dc.identifier.eissn | 1460-2202 | - |
dc.identifier.pmid | 27044350 | - |
dc.identifier.url | http://www.tandfonline.com/doi/abstract/10.3109/00365521.2015.1121513 | - |
dc.contributor.alternativeName | Kim, Chan Yun | - |
dc.contributor.alternativeName | Seong, Gong Je | - |
dc.contributor.alternativeName | Lee, Kwanghyun | - |
dc.contributor.alternativeName | Hong, Sa Min | - |
dc.contributor.affiliatedAuthor | Seong, Gong Je | - |
dc.contributor.affiliatedAuthor | Lee, Kwanghyun | - |
dc.contributor.affiliatedAuthor | Hong, Sa Min | - |
dc.contributor.affiliatedAuthor | Kim, Chan Yun | - |
dc.citation.volume | 41 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1367 | - |
dc.citation.endPage | 1372 | - |
dc.identifier.bibliographicCitation | CURRENT EYE RESEARCH, Vol.41(10) : 1367-1372, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 39645 | - |
dc.type.rims | ART | - |
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