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Cited 3 times in

Hypermethylation and downregulation of glutathione peroxidase 3 are related to pathogenesis of melanoma

Authors
 Haiyue Chen  ;  Zhenlong Zheng  ;  Ki-Yeol Kim  ;  Xuemei Jin  ;  Mi Ryung Roh  ;  Zhehu Jin 
Citation
 Oncology Reports, Vol.36(5) : 2737-2744, 2016 
Journal Title
 Oncology Reports 
ISSN
 1021-335X 
Issue Date
2016
MeSH
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Azacitidine/administration & dosage ; Azacitidine/analogs & derivatives ; Child ; DNA Methylation/drug effects ; DNA Methylation/genetics* ; Female ; Gene Expression Regulation, Neoplastic/drug effects ; Glutathione Peroxidase/biosynthesis ; Glutathione Peroxidase/genetics* ; Humans ; Male ; Melanoma/drug therapy ; Melanoma/genetics* ; Melanoma/pathology ; Middle Aged ; Promoter Regions, Genetic/genetics ; RNA, Messenger/biosynthesis
Abstract
As a crucial antioxidant enzyme, glutathione peroxidase 3 (GPX3) has been found to be frequently repressed in many cancers due to promoter hypermethylation and is known as a possible tumor suppressor gene. In the present study, we investigated whether promoter hypermethylation of GPX3 and its repression are present in melanoma and, if so, whether GPX3 downregulation is implicated in the pathogenesis of melanoma. Our results revealed methylation of GPX3 and downregulation of its expression in both melanoma cell lines and surgical melanoma tissue samples. In melanoma cell lines, GPX3 expression was restored by treatment with 5-aza-2'-deoxycytidine both in mRNA and protein levels. Depletion of GPX3 was found to increase the proliferative ability, motility, and invasiveness of melanoma cells. Moreover, negative expression of GPX3 was related to poor prognosis in melanoma patients. These results suggest that methylation-mediated GPX3 repression may have critical implications for melanoma pathogenesis.
Full Text
https://www.spandidos-publications.com/or/36/5/2737
DOI
10.3892/or.2016.5071
Appears in Collections:
1. Journal Papers (연구논문) > 5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실)
Yonsei Authors
김기열(Kim, Ki Yeol) ORCID logo https://orcid.org/0000-0001-5357-1067
노미령(Roh, Mi Ryung) ORCID logo https://orcid.org/0000-0002-6285-2490
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/152610
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