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Thymic stromal lymphopoietin regulates eosinophil migration via phosphorylation of l-plastin in atopic dermatitis

Authors
 Ji Yeon Noh  ;  Jung U Shin  ;  Chang Ook Park  ;  Nara Lee  ;  Shan Jin  ;  Seo Hyeong Kim  ;  Ji Hye Kim  ;  Arim Min  ;  Myeong Heon Shin  ;  Kwang Hoon Lee 
Citation
 EXPERIMENTAL DERMATOLOGY, Vol.25(11) : 880-886, 2016 
Journal Title
 EXPERIMENTAL DERMATOLOGY 
ISSN
 0906-6705 
Issue Date
2016
MeSH
Case-Control Studies ; Cell Line, Tumor ; Cytokines/metabolism* ; Dermatitis, Atopic/immunology* ; Dermatitis, Atopic/metabolism ; Eosinophils/metabolism* ; Humans ; Membrane Glycoproteins/metabolism* ; Microfilament Proteins/metabolism* ; Phosphorylation
Keywords
atopic dermatitis ; eosinophil ; migration ; phospho-l-plastin ; thymic stromal lymphopoietin
Abstract
Infiltration of eosinophils in atopic dermatitis (AD), which contains inflammatory molecules and cytokines, recruits more inflammatory cells and causes further skin damage. Thymic stromal lymphopoietin (TSLP) is an epithelial cytokine that induces the proinflammatory Th2 immune response and plays an important role in allergic disease. In this study, we aimed to identify a novel protein that regulates TSLP in eosinophils to further understand the role of eosinophils in atopic dermatitis. Using a proteomics approach, we identified the TSLP-inducible protein l-plastin and confirmed upregulation of l-plastin and p-l-plastin in TSLP-treated human eosinophilic leukaemic (EoL-1) cells and in eosinophils from AD patients. Migration assays showed that migration of eosinophils increased when cells were treated with TSLP and when cells were treated with TSLP and an additional cytokine such as interleukin (IL)-3, IL-4, IL-5 or IL-13, when compared to migration of untreated eosinophils. We also confirmed a positive correlation between phosphorylation of l-plastin and an increase in migration of TSLP and cytokine-treated eosinophils. In addition, phosphorylation of l-plastin was sensitive to PKCβII inhibition. Our results suggest that TSLP-induced phosphorylation of l-plastin affects eosinophil migration, which may be mediated by the protein kinase C signalling pathway in atopic dermatitis, thus suggesting p-l-plastin as a potential drug target for eosinophil-targeted allergy therapy.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/exd.13111/abstract
DOI
10.1111/exd.13111
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Noh, Ji Yeon(노지연)
Min, A Rim(민아림) ORCID logo https://orcid.org/0000-0003-2938-9630
Park, Chang Ook(박창욱) ORCID logo https://orcid.org/0000-0003-3856-1201
Shin, Myeong Heon(신명헌) ORCID logo https://orcid.org/0000-0001-8207-6110
Shin, Jung U(신정우) ORCID logo https://orcid.org/0000-0001-5259-6879
Lee, Kwang Hoon(이광훈)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/152545
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