Cited 30 times in
ZNF224, Kr?ppel like zinc finger protein, induces cell growth and apoptosis-resistance by down-regulation of p21 and p53 via miR-663a
DC Field | Value | Language |
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dc.contributor.author | 박세호 | - |
dc.contributor.author | 송승용 | - |
dc.date.accessioned | 2017-10-26T07:31:03Z | - |
dc.date.available | 2017-10-26T07:31:03Z | - |
dc.date.issued | 2016 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/152134 | - |
dc.description.abstract | ZNF224 is a Kr?ppel-associated box-containing zinc-finger protein which represses gene transcription by interacting with various co-repressors. However, its consensus DNA sequences and target genes are not fully identified. In this study, we identified and characterized consensus DNA sequences containing 5'-CAGC-3' recognized by ZNF224 through ChIP-sequencing, which further confirmed by ELISA, SPR, qPCR, and luciferase activity assay. ZNF224 increased miR-663a transcription by binding to miR-663a promoter, which in turn binds to 3' UTR of p53 and p21 to decrease their expression. miR-663a antagonist abolished ZNF224-mediated suppression of p21 and p53, resulting in the enhanced apoptosis by CPT. The analyses using human breast ductal carcinoma tissues exhibited that the expression of ZNF224 and miR-663a was increased in cancer compared to non-cancer region. Consequently, ZNF224 increases cell survival and decreases apoptosis by decreasing the expression of p53 and p21 via miR-663a as a transcriptional activator. Taken together, we identified and characterized DNA binding element of ZNF224, and its target genes, miR-663a, which provides a novel insight in the down-regulation of p21 and p53 via miR-663a by ZNF224 in breast cancer. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Impact Journals | - |
dc.relation.isPartOf | ONCOTARGET | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Apoptosis/physiology | - |
dc.subject.MESH | Breast Neoplasms/genetics | - |
dc.subject.MESH | Breast Neoplasms/metabolism* | - |
dc.subject.MESH | Breast Neoplasms/pathology | - |
dc.subject.MESH | Carcinoma, Ductal, Breast/genetics | - |
dc.subject.MESH | Carcinoma, Ductal, Breast/metabolism* | - |
dc.subject.MESH | Carcinoma, Ductal, Breast/pathology | - |
dc.subject.MESH | Cell Cycle/physiology | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cell Proliferation/physiology | - |
dc.subject.MESH | Cyclin-Dependent Kinase Inhibitor p21/genetics | - |
dc.subject.MESH | Cyclin-Dependent Kinase Inhibitor p21/metabolism* | - |
dc.subject.MESH | Down-Regulation | - |
dc.subject.MESH | Genes, p53 | - |
dc.subject.MESH | HEK293 Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | MCF-7 Cells | - |
dc.subject.MESH | MicroRNAs/genetics | - |
dc.subject.MESH | MicroRNAs/metabolism* | - |
dc.subject.MESH | Repressor Proteins/genetics | - |
dc.subject.MESH | Repressor Proteins/metabolism* | - |
dc.subject.MESH | Transfection | - |
dc.subject.MESH | Tumor Suppressor Protein p53/genetics | - |
dc.subject.MESH | Tumor Suppressor Protein p53/metabolism* | - |
dc.title | ZNF224, Kr?ppel like zinc finger protein, induces cell growth and apoptosis-resistance by down-regulation of p21 and p53 via miR-663a | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Surgery | - |
dc.contributor.googleauthor | Jin Gu Cho | - |
dc.contributor.googleauthor | Seho Park | - |
dc.contributor.googleauthor | Chae Hyun Lim | - |
dc.contributor.googleauthor | Hong Sook Kim | - |
dc.contributor.googleauthor | Seung Yong Song | - |
dc.contributor.googleauthor | Tae-Young Roh | - |
dc.contributor.googleauthor | Jong-Hyuk Sung | - |
dc.contributor.googleauthor | Wonhee Suh | - |
dc.contributor.googleauthor | Seok-Jin Ham | - |
dc.contributor.googleauthor | Key-Hwan Lim | - |
dc.contributor.googleauthor | Sang Gyu Park | - |
dc.identifier.doi | 10.18632/oncotarget.8870 | - |
dc.contributor.localId | A02032 | - |
dc.contributor.localId | A01524 | - |
dc.relation.journalcode | J02421 | - |
dc.identifier.eissn | 1949-2553 | - |
dc.identifier.pmid | 27105517 | - |
dc.subject.keyword | ZNF224 | - |
dc.subject.keyword | apoptosis | - |
dc.subject.keyword | miR-663a | - |
dc.subject.keyword | p21 | - |
dc.subject.keyword | p53 | - |
dc.contributor.alternativeName | Park, Se Ho | - |
dc.contributor.alternativeName | Song, Seung Yong | - |
dc.contributor.affiliatedAuthor | Song, Seung Yong | - |
dc.contributor.affiliatedAuthor | Park, Se Ho | - |
dc.citation.volume | 7 | - |
dc.citation.number | 21 | - |
dc.citation.startPage | 31177 | - |
dc.citation.endPage | 31190 | - |
dc.identifier.bibliographicCitation | ONCOTARGET , Vol.7(21) : 31177-31190, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 46913 | - |
dc.type.rims | ART | - |
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