Cited 7 times in
Generation of ΔF508-CFTR T84 cell lines by CRISPR/Cas9-mediated genome editing
DC Field | Value | Language |
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dc.contributor.author | 김주영 | - |
dc.contributor.author | 김형범 | - |
dc.contributor.author | 이민구 | - |
dc.date.accessioned | 2017-10-26T07:25:47Z | - |
dc.date.available | 2017-10-26T07:25:47Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/152015 | - |
dc.description.abstract | OBJECTIVES: To provide a simple method to make a stable ΔF508-CFTR-expressing T84 cell line that can be used as an efficient screening model system for ΔF508-CFTR rescue. RESULTS: CFTR knockout cell lines were generated by Cas9 with a single-guide RNA (sgRNA) targeting exon 1 of the CFTR genome, which produced indels that abolished CFTR protein expressions. Next, stable ΔF508-CFTR expression was achieved by genome integration of ΔF508-CFTR via the lentivirus infection system. Finally, we showed functional rescue of ΔF508-CFTR not only by growing the cells at a low temperature, but also incubating with VX-809, a ΔF508-CFTR corrector, in the established T84 cells expressing ΔF508-CFTR. CONCLUSIONS: This cell system provides an appropriate screening platform for rescue of ΔF508-CFTR, especially related to protein folding, escaped from endoplasmic-reticulum-associated protein degradation, and membrane transport. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.publisher | Kluwer Academic Publishers | - |
dc.relation.isPartOf | BIOTECHNOLOGY LETTERS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Clustered Regularly Interspaced Short Palindromic Repeats/genetics | - |
dc.subject.MESH | Endoplasmic Reticulum/metabolism | - |
dc.subject.MESH | Flow Cytometry | - |
dc.subject.MESH | Gene Editing/methods* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mutation | - |
dc.title | Generation of ΔF508-CFTR T84 cell lines by CRISPR/Cas9-mediated genome editing | - |
dc.type | Article | - |
dc.publisher.location | Netherlands | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Pharmacology | - |
dc.contributor.googleauthor | Woo Young Chung | - |
dc.contributor.googleauthor | Myungjae Song | - |
dc.contributor.googleauthor | Jinhong Park | - |
dc.contributor.googleauthor | Wan Namkung | - |
dc.contributor.googleauthor | Jinu Lee | - |
dc.contributor.googleauthor | Hyongbum Kim | - |
dc.contributor.googleauthor | Min Goo Lee | - |
dc.contributor.googleauthor | Joo Young Kim | - |
dc.identifier.doi | 10.1007/s10529-016-2190-4 | - |
dc.contributor.localId | A01148 | - |
dc.contributor.localId | A02781 | - |
dc.contributor.localId | A00942 | - |
dc.relation.journalcode | J00336 | - |
dc.identifier.eissn | 1573-6776 | - |
dc.identifier.pmid | 27571970 | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs10529-016-2190-4 | - |
dc.subject.keyword | CRISPR/Cas9 | - |
dc.subject.keyword | Epithelial cell | - |
dc.subject.keyword | Genome editing | - |
dc.subject.keyword | T84 cell line | - |
dc.subject.keyword | ΔF508-CFTR | - |
dc.contributor.alternativeName | Kim, Joo Young | - |
dc.contributor.alternativeName | Kim, Hyongbum | - |
dc.contributor.alternativeName | Lee, Min Goo | - |
dc.contributor.affiliatedAuthor | Kim, Hyongbum | - |
dc.contributor.affiliatedAuthor | Lee, Min Goo | - |
dc.contributor.affiliatedAuthor | Kim, Joo Young | - |
dc.citation.volume | 38 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 2023 | - |
dc.citation.endPage | 2034 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY LETTERS, Vol.38(12) : 2023-2034, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 46337 | - |
dc.type.rims | ART | - |
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