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Generation of ΔF508-CFTR T84 cell lines by CRISPR/Cas9-mediated genome editing

DC FieldValueLanguage
dc.contributor.author김주영-
dc.contributor.author김형범-
dc.contributor.author이민구-
dc.date.accessioned2017-10-26T07:25:47Z-
dc.date.available2017-10-26T07:25:47Z-
dc.date.issued2016-
dc.identifier.issn0141-5492-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/152015-
dc.description.abstractOBJECTIVES: To provide a simple method to make a stable ΔF508-CFTR-expressing T84 cell line that can be used as an efficient screening model system for ΔF508-CFTR rescue. RESULTS: CFTR knockout cell lines were generated by Cas9 with a single-guide RNA (sgRNA) targeting exon 1 of the CFTR genome, which produced indels that abolished CFTR protein expressions. Next, stable ΔF508-CFTR expression was achieved by genome integration of ΔF508-CFTR via the lentivirus infection system. Finally, we showed functional rescue of ΔF508-CFTR not only by growing the cells at a low temperature, but also incubating with VX-809, a ΔF508-CFTR corrector, in the established T84 cells expressing ΔF508-CFTR. CONCLUSIONS: This cell system provides an appropriate screening platform for rescue of ΔF508-CFTR, especially related to protein folding, escaped from endoplasmic-reticulum-associated protein degradation, and membrane transport.-
dc.description.statementOfResponsibilityrestriction-
dc.publisherKluwer Academic Publishers-
dc.relation.isPartOfBIOTECHNOLOGY LETTERS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHCell Line-
dc.subject.MESHClustered Regularly Interspaced Short Palindromic Repeats/genetics-
dc.subject.MESHEndoplasmic Reticulum/metabolism-
dc.subject.MESHFlow Cytometry-
dc.subject.MESHGene Editing/methods*-
dc.subject.MESHHumans-
dc.subject.MESHMutation-
dc.titleGeneration of ΔF508-CFTR T84 cell lines by CRISPR/Cas9-mediated genome editing-
dc.typeArticle-
dc.publisher.locationNetherlands-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Pharmacology-
dc.contributor.googleauthorWoo Young Chung-
dc.contributor.googleauthorMyungjae Song-
dc.contributor.googleauthorJinhong Park-
dc.contributor.googleauthorWan Namkung-
dc.contributor.googleauthorJinu Lee-
dc.contributor.googleauthorHyongbum Kim-
dc.contributor.googleauthorMin Goo Lee-
dc.contributor.googleauthorJoo Young Kim-
dc.identifier.doi10.1007/s10529-016-2190-4-
dc.contributor.localIdA01148-
dc.contributor.localIdA02781-
dc.contributor.localIdA00942-
dc.relation.journalcodeJ00336-
dc.identifier.eissn1573-6776-
dc.identifier.pmid27571970-
dc.identifier.urlhttps://link.springer.com/article/10.1007%2Fs10529-016-2190-4-
dc.subject.keywordCRISPR/Cas9-
dc.subject.keywordEpithelial cell-
dc.subject.keywordGenome editing-
dc.subject.keywordT84 cell line-
dc.subject.keywordΔF508-CFTR-
dc.contributor.alternativeNameKim, Joo Young-
dc.contributor.alternativeNameKim, Hyongbum-
dc.contributor.alternativeNameLee, Min Goo-
dc.contributor.affiliatedAuthorKim, Hyongbum-
dc.contributor.affiliatedAuthorLee, Min Goo-
dc.contributor.affiliatedAuthorKim, Joo Young-
dc.citation.volume38-
dc.citation.number12-
dc.citation.startPage2023-
dc.citation.endPage2034-
dc.identifier.bibliographicCitationBIOTECHNOLOGY LETTERS, Vol.38(12) : 2023-2034, 2016-
dc.date.modified2017-10-24-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

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