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Aromatase inhibitor regulates let-7 expression and let-7f-induced cell migration in endometrial cells from women with endometriosis

Authors
 SiHyun Cho  ;  Levent Mutlu  ;  Yuping Zhou  ;  Hugh S. Taylor 
Citation
 FERTILITY AND STERILITY, Vol.106(3) : 673-680, 2016 
Journal Title
 FERTILITY AND STERILITY 
ISSN
 0015-0282 
Issue Date
2016
MeSH
Aromatase/metabolism* ; Aromatase Inhibitors/pharmacology* ; Cell Line ; Cell Movement/drug effects* ; Dose-Response Relationship, Drug ; Endometriosis/enzymology* ; Endometriosis/genetics ; Endometriosis/pathology ; Endometriosis/surgery ; Endometrium/drug effects* ; Endometrium/enzymology ; Endometrium/pathology ; Endometrium/surgery ; Estradiol/pharmacology ; Female ; Humans ; Laparoscopy ; MicroRNAs/genetics ; MicroRNAs/metabolism* ; Nitriles/pharmacology* ; Signal Transduction/drug effects ; Stromal Cells/drug effects* ; Stromal Cells/enzymology ; Stromal Cells/pathology ; Time Factors ; Transfection ; Triazoles/pharmacology*
Keywords
Aromatase inhibitor ; endometriosis ; let-7f ; microRNA
Abstract
OBJECTIVE: To evaluate associations between aromatase inhibitor (AI) treatment and let-7-family microRNA expression in endometriosis. DESIGN: In vitro study with the use of Ishikawa cells and human endometrial stromal cells (HESCs) obtained from patients with endometriosis. SETTING: University research center. PATIENT(S): Women undergoing laparoscopic surgery for endometriosis. INTERVENTION(S): HESCs and Ishikawa cells treated with various letrozole concentrations and transfected with a mimic of let-7 subtypes of interest. MAIN OUTCOME MEASURE(S): MicroRNAs let-7a-f and aromatase expression were evaluated. Migration potential after transfection with a let-7f mimic were analyzed. RESULT(S): After letrozole treatment for 48 hours, all let-7 subtypes showed a trend toward increased expression in a dose-dependent manner in Ishikawa cells, and significant differences were found in let-7b and let-7f between the control and 20 μmol/L treatment groups. Furthermore, let-7f showed significant differences between the control group and 1.0 μmol/L treatment group, a typical therapeutic level, in HESCs. Transfection of a let-7f mimic decreased aromatase expression in both Ishikawa cells and HESCs and led to a significant decrease in number of migrating cells in both cell types. CONCLUSION(S): AI treatment significantly increased expression of let-7f in Ishikawa cells and HESCs from patients with endometriosis; increased let-7f expression effectively reduced the migration of endometrial cells. Modulation of microRNAs involved in the pathogenesis of endometriosis may have therapeutic potential for endometriosis.
Full Text
http://www.sciencedirect.com/science/article/pii/S0015028216612876?via%3Dihub
DOI
10.1016/j.fertnstert.2016.05.020
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
Yonsei Authors
Cho, Si Hyun(조시현) ORCID logo https://orcid.org/0000-0003-2718-6645
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151970
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