79 40

Cited 60 times in

Multiplexed and scalable super-resolution imaging of three-dimensional protein localization in size-adjustable tissues

Authors
 Taeyun Ku  ;  Justin Swaney  ;  Jeong-Yoon Park  ;  Alexandre Albanese  ;  Evan Murray  ;  Jae Hun Cho  ;  Young-Gyun Park  ;  Vamsi Mangena  ;  Jiapei Chen  ;  Kwanghun Chung 
Citation
 Nature Biotechnology, Vol.34(9) : 973-981, 2016 
Journal Title
 Nature Biotechnology 
ISSN
 1087-0156 
Issue Date
2016
MeSH
Animals ; Brain/metabolism* ; Brain/ultrastructure ; Female ; Gene Expression Profiling/methods ; Image Enhancement/methods ; Image Interpretation, Computer-Assisted/methods ; Imaging, Three-Dimensional/methods* ; Immunoassay/methods ; Male ; Mice ; Molecular Imaging/methods* ; Nerve Tissue Proteins/metabolism ; Proteome/metabolism* ; Proteome/ultrastructure ; Synapses/metabolism* ; Synapses/ultrastructure* ; Tissue Distribution
Abstract
The biology of multicellular organisms is coordinated across multiple size scales, from the subnanoscale of molecules to the macroscale, tissue-wide interconnectivity of cell populations. Here we introduce a method for super-resolution imaging of the multiscale organization of intact tissues. The method, called magnified analysis of the proteome (MAP), linearly expands entire organs fourfold while preserving their overall architecture and three-dimensional proteome organization. MAP is based on the observation that preventing crosslinking within and between endogenous proteins during hydrogel-tissue hybridization allows for natural expansion upon protein denaturation and dissociation. The expanded tissue preserves its protein content, its fine subcellular details, and its organ-scale intercellular connectivity. We use off-the-shelf antibodies for multiple rounds of immunolabeling and imaging of a tissue's magnified proteome, and our experiments demonstrate a success rate of 82% (100/122 antibodies tested). We show that specimen size can be reversibly modulated to image both inter-regional connections and fine synaptic architectures in the mouse brain.
Files in This Item:
T201603410.pdf Download
DOI
10.1038/nbt.3641
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Park, Jeong Yoon(박정윤) ORCID logo https://orcid.org/0000-0002-3728-7784
Export
RIS (EndNote)
XLS (Excel)
XML
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151940
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse