Cited 37 times in
Role of miR-146a in the Regulation of Inflammation in an In Vitro Model of Graves' Orbitopathy
DC Field | Value | Language |
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dc.contributor.author | 윤진숙 | - |
dc.contributor.author | 이은직 | - |
dc.date.accessioned | 2017-10-26T07:21:35Z | - |
dc.date.available | 2017-10-26T07:21:35Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0146-0404 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/151927 | - |
dc.description.abstract | PURPOSE: To investigate the role of microRNA 146a (miR-146a) in the regulation of inflammation in an in vitro model of Graves' orbitopathy (GO). METHODS: The level of miR-146a expression in orbital adipose tissue was compared between GO and non-GO by quantitative real-time PCR (qPCR). The effects of interleukin 1β (IL-1β) on miR-146a expression were analyzed in orbital fibroblasts by qPCR. To investigate the molecular mechanism underlying IL-1β-induced miR-146a expression, the effects of inhibitors of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), mitogen-activated protein kinase/extracellular signal-regulated kinases (MEK)-1/2, c-Jun N-terminal kinases (JNK)-1/2, p38 MAP kinase, and phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) were analyzed. The effects of miR-146a mimics and inhibitors on IL-1β-induced IL-6 release were examined by ELISA and Western blotting. RESULTS: The level of miR-146a expression was significantly higher in GO orbital adipose tissue than in non-GO (P = 0.032). Interleukin 1β induced a time- and concentration-dependent increase in miR-146a expression. Interleukin 1β (10 ng/mL, 16 hours) induced an approximately 17.5-fold increase in miR-146 expression. The increase in miR-146a expression by IL-1β was significantly inhibited by NF-κB, JNK-1/2, and PI3K inhibitors (1.94? ± ?0.25, 5.28? ± ?0.34 and 9.73? ± ?2.32-fold, respectively, P < 0.05 compared with IL-1β-induced miR-146 expression, independent t-test). Interleukin 1β-induced IL-6 protein production was further decreased by miR-146a mimics, but not by inhibitors of miR-146a. CONCLUSIONS: MicroRNA 146a was upregulated by inflammatory stress in orbital fibroblasts. Our results indicated that miR-146a had a positive effect on the anti-inflammatory process. MicroRNA 146a may play a role in the regulation of inflammation in orbital fibroblasts, and may participate in the pathogenesis of GO. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Association For Research In Vision And Ophthalmology (Arvo) | - |
dc.relation.isPartOf | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Enzyme-Linked Immunosorbent Assay | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Fibroblasts/metabolism | - |
dc.subject.MESH | Fibroblasts/pathology | - |
dc.subject.MESH | Gene Expression Regulation* | - |
dc.subject.MESH | Graves Ophthalmopathy/genetics* | - |
dc.subject.MESH | Graves Ophthalmopathy/metabolism | - |
dc.subject.MESH | Graves Ophthalmopathy/pathology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Inflammation/genetics | - |
dc.subject.MESH | Inflammation/metabolism* | - |
dc.subject.MESH | Inflammation/pathology | - |
dc.subject.MESH | Interleukin-6/metabolism | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | MicroRNAs/biosynthesis | - |
dc.subject.MESH | MicroRNAs/genetics* | - |
dc.subject.MESH | Microarray Analysis | - |
dc.subject.MESH | RNA/genetics* | - |
dc.subject.MESH | Real-Time Polymerase Chain Reaction | - |
dc.title | Role of miR-146a in the Regulation of Inflammation in an In Vitro Model of Graves' Orbitopathy | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Ophthalmology | - |
dc.contributor.googleauthor | Sun Young Jang | - |
dc.contributor.googleauthor | Min Kyung Chae | - |
dc.contributor.googleauthor | Joon H. Lee | - |
dc.contributor.googleauthor | Eun Jig Lee | - |
dc.contributor.googleauthor | Jin Sook Yoon | - |
dc.identifier.doi | 10.1167/iovs.16-19213 | - |
dc.contributor.localId | A03050 | - |
dc.contributor.localId | A02611 | - |
dc.relation.journalcode | J01187 | - |
dc.identifier.eissn | 1552-5783 | - |
dc.identifier.pmid | 27494344 | - |
dc.identifier.url | http://iovs.arvojournals.org/article.aspx?articleid=2543210 | - |
dc.subject.keyword | miR-146a | - |
dc.subject.keyword | inflammation | - |
dc.subject.keyword | Graves’ orbitopathy | - |
dc.contributor.alternativeName | Yoon, Jin Sook | - |
dc.contributor.alternativeName | Lee, Eun Jig | - |
dc.contributor.affiliatedAuthor | Lee, Eun Jig | - |
dc.contributor.affiliatedAuthor | Yoon, Jin Sook | - |
dc.citation.volume | 57 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 4027 | - |
dc.citation.endPage | 4034 | - |
dc.identifier.bibliographicCitation | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol.57(10) : 4027-4034, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 46250 | - |
dc.type.rims | ART | - |
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