0 162

Cited 2 times in

Magnetic bead-based nucleic acid purification kit: Clinical application and performance evaluation in stool specimens

Authors
 Jihoon G. Yoon  ;  Jin Seok Kang  ;  Seung Yong Hwang  ;  Jaewoo Song  ;  Seok Hoon Jeong 
Citation
 Journal of Microbiological Methods, Vol.124 : 62-68, 2016 
Journal Title
 Journal of Microbiological Methods 
ISSN
 0167-7012 
Issue Date
2016
MeSH
Automation ; Bacteria/classification ; Bacteria/genetics ; Bacteria/isolation & purification* ; Bacterial Infections/diagnosis ; Bacterial Infections/microbiology* ; DNA, Bacterial/genetics ; DNA, Bacterial/isolation & purification* ; Feces/microbiology* ; Humans ; Magnetics/instrumentation ; Magnetics/methods* ; Polymerase Chain Reaction ; Reagent Kits, Diagnostic
Keywords
Clostridium difficile ; Nucleic acid extraction ; Rotavirus ; Stool
Abstract
Two different methods - the semi-automated magnetic bead-based kit (SK, Stool DNA/RNA Purification kit®) and the manual membrane column-based kit (QS, QIAamp® DNA Stool Mini kit) - for purifying nucleic acids from clinical stool samples were compared and evaluated. The SK kit was more user-friendly than QS due to the reduced manual processing, partial automation, and short turnaround time with half cost. Furthermore, SK produced high yields in both DNA and RNA extractions but poor purity in RNA extraction. In the assessment of rotavirus and Clostridium difficile infection, both kits had equivalent or more sensitive performance compared with the standard method. Although SK showed some interference and inhibition in nucleic acid extraction, the performance, including the repeatability, linearity, analytical sensitivity, and matrix effect, was sufficient for routine clinical use.
Full Text
http://www.sciencedirect.com/science/article/pii/S0167701216300458
DOI
10.1016/j.mimet.2016.03.011
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
송재우(Song, Jae Woo) ORCID logo https://orcid.org/0000-0002-1877-5731
윤지훈(Yoon, Jihoon G.) ORCID logo https://orcid.org/0000-0002-4401-7803
정석훈(Jeong, Seok Hoon) ORCID logo https://orcid.org/0000-0001-9290-897X
Export
RIS (EndNote)
XLS (Excel)
XML
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151685
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse