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The Resistance Mechanism and Clonal Distribution of Tigecycline-Nonsusceptible Klebsiella pneumoniae Isolates in Korea

DC Field Value Language
dc.contributor.author용태순-
dc.contributor.author윤상선-
dc.contributor.author이경원-
dc.contributor.author정석훈-
dc.date.accessioned2017-10-26T07:09:47Z-
dc.date.available2017-10-26T07:09:47Z-
dc.date.issued2016-
dc.identifier.issn0513-5796-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/151683-
dc.description.abstractPURPOSE: Tigecycline is one of the drugs used to treat multi-drug resistant Klebsiella pneumoniae (K. pneumoniae) infections, including complicated skin and soft tissue infections, complicated intra-abdominal infection, and community-acquired pneumonia in the Republic of Korea. However, since its commercial release, K. pneumoniae resistance against tigecycline has been reported, and there is a serious concern about the spread of tigecycline resistant bacteria. MATERIALS AND METHODS: In this study, we collected and analyzed 342 isolates from 23 hospitals in the Republic of Korea to determine the mechanisms of tigecycline susceptibility and their clonal types. The hospitals include several from each province in the Republic of Korea, except Jeju, an island province, and nonsusceptibility among the isolates was tested by the disk diffusion method. In our lab, susceptibility was checked again using the broth dilution method, and clonal types were determined using the multilocus sequence typing protocol. Real-time PCR was performed to measure the ramR mutation in the isolates nonsusceptible to tigecycline, which would suggest an increased expression of the AcrAB multidrug pump. RESULTS: Fifty-six K. pneumoniae isolates were found to be nonsusceptible, 16% of the 342 collected. Twenty-seven and nine isolates of the tigecycline nonsusceptible isolates had mutations in the ramR and rpsJ genes, respectively; while 18 nonsusceptible isolates harbored the tetA gene. Comparison of isolates with and without ramR mutation showed a significant statistical difference (p<0.05) for expression of AcrAB. Moreover, the most common clonal types, as observed in our study, appear to be ST11 and ST789. CONCLUSION: Several dominate clonal types infer tigecycline resistance to K. pneumoniae, including ST11, ST768, ST15, ST23, ST48, and ST307. There does not seem to be a transferrable medium, such as plasmid, for the resistance yet, although mutation of the ramR gene may be a common event, accounting for 48% of the nonsusceptibility in this study.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.languageEnglish-
dc.publisherYonsei University-
dc.relation.isPartOfYONSEI MEDICAL JOURNAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnti-Bacterial Agents/pharmacology*-
dc.subject.MESHAnti-Bacterial Agents/therapeutic use-
dc.subject.MESHBacterial Proteins-
dc.subject.MESHDrug Resistance, Bacterial*-
dc.subject.MESHHumans-
dc.subject.MESHKlebsiella Infections/drug therapy*-
dc.subject.MESHKlebsiella pneumoniae/drug effects*-
dc.subject.MESHKlebsiella pneumoniae/genetics-
dc.subject.MESHKlebsiella pneumoniae/isolation & purification*-
dc.subject.MESHMicrobial Sensitivity Tests-
dc.subject.MESHMinocycline/analogs & derivatives*-
dc.subject.MESHMinocycline/pharmacology-
dc.subject.MESHMinocycline/therapeutic use-
dc.subject.MESHMultilocus Sequence Typing-
dc.subject.MESHPlasmids-
dc.subject.MESHPolymerase Chain Reaction-
dc.subject.MESHReal-Time Polymerase Chain Reaction-
dc.subject.MESHRepublic of Korea-
dc.titleThe Resistance Mechanism and Clonal Distribution of Tigecycline-Nonsusceptible Klebsiella pneumoniae Isolates in Korea-
dc.typeArticle-
dc.publisher.locationKorea (South)-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Environmental Medical Biology-
dc.contributor.googleauthorChulsoo Ahn-
dc.contributor.googleauthorSang Sun Yoon-
dc.contributor.googleauthorTae Soon Yong-
dc.contributor.googleauthorSeok Hoon Jeong-
dc.contributor.googleauthorKyungwon Lee-
dc.identifier.doi10.3349/ymj.2016.57.3.641-
dc.contributor.localIdA02558-
dc.contributor.localIdA02649-
dc.contributor.localIdA03619-
dc.contributor.localIdA02424-
dc.relation.journalcodeJ02813-
dc.identifier.eissn1976-2437-
dc.identifier.pmid26996563-
dc.subject.keywordKlebsiella pneumoniae-
dc.subject.keywordTigecycline-
dc.subject.keywordramR-
dc.contributor.alternativeNameYong, Tai Soon-
dc.contributor.alternativeNameYoon, Sang Sun-
dc.contributor.alternativeNameLee, Kyung Won-
dc.contributor.alternativeNameJeong, Seok Hoon-
dc.contributor.affiliatedAuthorYoon, Sang Sun-
dc.contributor.affiliatedAuthorLee, Kyung Won-
dc.contributor.affiliatedAuthorJeong, Seok Hoon-
dc.contributor.affiliatedAuthorYong, Tai Soon-
dc.citation.volume57-
dc.citation.number3-
dc.citation.startPage641-
dc.citation.endPage646-
dc.identifier.bibliographicCitationYONSEI MEDICAL JOURNAL, Vol.57(3) : 641-646, 2016-
dc.date.modified2017-10-24-
dc.identifier.rimsid45697-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers

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