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Identification of Acinetobacter Species Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

 Seri Jeong  ;  Jun Sung Hong  ;  Jung Ok Kim  ;  Keon-Han Kim  ;  Woonhyoung Lee  ;  Il Kwon Bae  ;  Kyungwon Lee  ;  Seok Hoon Jeong 
 Annals of Laboratory Medicine, Vol.36(4) : 325-334, 2016 
Journal Title
 Annals of Laboratory Medicine 
Issue Date
Acinetobacter Infections/microbiology* ; Acinetobacter Infections/pathology ; Acinetobacter baumannii/chemistry* ; Acinetobacter baumannii/classification ; Acinetobacter baumannii/isolation & purification ; Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Databases, Factual ; Humans ; Phylogeny ; RNA, Ribosomal, 16S/chemistry ; RNA, Ribosomal, 16S/genetics ; RNA, Ribosomal, 16S/metabolism ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*
Acinetobacter ; Database ; Identification ; MALDI-TOF MS ; Species
BACKGROUND: Acinetobacter baumannii has a greater clinical impact and exhibits higher antimicrobial resistance rates than the non-baumannii Acinetobacter species. Therefore, the correct identification of Acinetobacter species is clinically important. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has recently become the method of choice for identifying bacterial species. The purpose of this study was to evaluate the ability of MALDI-TOF MS (Bruker Daltonics GmbH, Germany) in combination with an improved database to identify various Acinetobacter species. METHODS: A total of 729 Acinetobacter clinical isolates were investigated, including 447 A. baumannii, 146 A. nosocomialis, 78 A. pittii, 18 A. ursingii, 9 A. bereziniae, 9 A. soli, 4 A. johnsonii, 4 A. radioresistens, 3 A. gyllenbergii, 3 A. haemolyticus, 2 A. lwoffii, 2 A. junii, 2 A. venetianus, and 2 A. genomospecies 14TU. After 212 isolates were tested with the default Bruker database, the profiles of 63 additional Acinetobacter strains were added to the default database, and 517 isolates from 32 hospitals were assayed for validation. All strains in this study were confirmed by rpoB sequencing. RESULTS: The addition of the 63 Acinetobacter strains' profiles to the default Bruker database increased the overall concordance rate between MALDI-TOF MS and rpoB sequencing from 69.8% (148/212) to 100.0% (517/517). Moreover, after library modification, all previously mismatched 64 Acinetobacter strains were correctly identified. CONCLUSIONS: MALDI-TOF MS enables the prompt and accurate identification of clinically significant Acinetobacter species when used with the improved database.
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
김건한(Kim, Keon-Han)
이경원(Lee, Kyungwon) ORCID logo https://orcid.org/0000-0003-3788-2134
정석훈(Jeong, Seok Hoon) ORCID logo https://orcid.org/0000-0001-9290-897X
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