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De novo osteogenesis from human ligamentum flavum by adenovirus-mediated bone morphogenetic protein-2 gene transfer
DC Field | Value | Language |
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dc.contributor.author | 김주항 | - |
dc.contributor.author | 문성환 | - |
dc.contributor.author | 박시영 | - |
dc.contributor.author | 양익환 | - |
dc.contributor.author | 윤채옥 | - |
dc.contributor.author | 이환모 | - |
dc.date.accessioned | 2017-10-26T06:56:47Z | - |
dc.date.available | 2017-10-26T06:56:47Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0362-2436 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/151525 | - |
dc.description.abstract | STUDY DESIGN: In vitro and in vivo experiment using degenerated human ligamentum flavum (LF) and Type 5 adenovirus construct with bone morphogenetic protein-2 (BMP-2) cDNA. OBJECTIVES: To demonstrate in vitro and in vivo osteogenic effect of BMP-2 gene transfer to human LF and to propose genetically modified LF as a substitute for autogenous bone graft in spinal fusion. SUMMARY OF BACKGROUND DATA: Spinal fusion is still considered to be an important option for treating various spinal disorders. To induce solid spinal fusion, osteoinductive and/or osteoconductive agents have been widely adopted. Autogenous LF, however, has never been seriously considered as a carrier for ex vivo osteoinductive gene therapy for spinal fusion. METHODS: In vitro experiment: Degenerated human LF was harvested and cultured. Type 5 adenovirus lacZ (Ad/lacZ) and BMP-2 construct (Ad/BMP-2) were produced. LF cell cultures were then exposed to Ad/BMP-2. Expressions of osteocalcin and BMP-2 mRNA were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR). Western blot analysis was performed to detect osteocalcin protein. Alkaline phosphatase and von Kossa stains were used to detect osteogenic markers and bone nodule formation, respectively. In vivo experiment: Human LF tissues treated with Ad/lacZ, Ad/BMP-2, and saline were implanted into the subcutaneous tissue of nude mice. After 4 weeks, nude mice were radiographed and killed. Implanted LF tissues were harvested and histologically stained. RESULTS: LF cell cultures with Ad/BMP-2 revealed strong expression of BMP-2 and osteocalcin mRNA in RT-PCR and osteocalcin protein in western blot analysis. LF cell culture with saline showed baseline expression of BMP-2, osteocalcin mRNA, and osteocalcin protein, respectively. Furthermore, LF cell culture with Ad/BMP-2 demonstrated the expression of alkaline phosphatase and bone nodule formation in the aforementioned histochemical stain. LF tissues with Ad/BMP-2 revealed de novo osteogenesis in nude mice, whereas LF with Ad/lacZ or saline showed only remaining LF tissue without sign of bone formation. CONCLUSION: Human LF cells transduced with Ad/BMP-2 exhibited the expression of osteogenic phenotype and bone nodule formation. Additionally, genetically modified human LF with BMP-2 cDNA clearly demonstrated de novo osteogenesis, which supports the concept that biologically modified LF can be a substitute for autogenous bone graft in spinal fusion surgery. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Lippincott Williams & Wilkins | - |
dc.relation.isPartOf | SPINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | De novo osteogenesis from human ligamentum flavum by adenovirus-mediated bone morphogenetic protein-2 gene transfer | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | Research Institutes (연구소) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.department | Dept. of Orthopedic Surgery (정형외과학교실) | - |
dc.contributor.department | Dept. of Orthopedic Surgery (정형외과학교실) | - |
dc.contributor.department | Dept. of Orthopedic Surgery (정형외과학교실) | - |
dc.contributor.department | Institute for Cancer Research (암연구소) | - |
dc.contributor.department | Dept. of Orthopedic Surgery (정형외과학교실) | - |
dc.contributor.googleauthor | Ick-Hwan Yang | - |
dc.contributor.googleauthor | Hyang Kim | - |
dc.contributor.googleauthor | Un-Hye Kwon | - |
dc.contributor.googleauthor | Kwang-Il Lee | - |
dc.contributor.googleauthor | Ji-Ae Jun | - |
dc.contributor.googleauthor | Joo-Hang Kim | - |
dc.contributor.googleauthor | Che-Ok Yun | - |
dc.contributor.googleauthor | Si-Young Park | - |
dc.contributor.googleauthor | Hwan-Mo Lee | - |
dc.contributor.googleauthor | Seong-Hwan Moon | - |
dc.identifier.doi | OAK-2005-06205 | - |
dc.contributor.localId | A00945 | - |
dc.contributor.localId | A01365 | - |
dc.contributor.localId | A01554 | - |
dc.contributor.localId | A02313 | - |
dc.contributor.localId | A02614 | - |
dc.contributor.localId | A03333 | - |
dc.relation.journalcode | J02674 | - |
dc.identifier.eissn | 1528-1159 | - |
dc.identifier.url | http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&AN=00007632-200512150-00008&LSLINK=80&D=ovft | - |
dc.contributor.alternativeName | Kim, Joo Hang | - |
dc.contributor.alternativeName | Kim, Joo Hang | - |
dc.contributor.alternativeName | Moon, Seong Hwan | - |
dc.contributor.alternativeName | Park, Si Young | - |
dc.contributor.alternativeName | Yang, Ick Hwan | - |
dc.contributor.alternativeName | Yun, Chae Ok | - |
dc.contributor.alternativeName | Lee, Hwan Mo | - |
dc.contributor.affiliatedAuthor | 김주항 | - |
dc.citation.volume | 30 | - |
dc.citation.number | 24 | - |
dc.citation.startPage | 2749 | - |
dc.citation.endPage | 2754 | - |
dc.identifier.bibliographicCitation | SPINE, Vol.30(24) : 2749-2754, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 44678 | - |
dc.type.rims | ART | - |
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