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Evaluation of phenotypic screening methods for detecting plasmid-mediated AmpC β-lactamases-producing isolates of Escherichia coli and Klebsiella pneumoniae

Authors
 Kyungwon Lee  ;  Seong Geun Hong  ;  Yeon Joon Park  ;  Hye Soo Lee  ;  Wonkeun Song  ;  Joseph Jeong  ;  Dongeun Yong  ;  Yunsop Chong 
Citation
 DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, Vol.53(4) : 319-323, 2005 
Journal Title
 DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE 
ISSN
 0732-8893 
Issue Date
2005
MeSH
Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/analysis* ; Bacterial Proteins/genetics ; Cefoxitin/pharmacology ; Enzyme Induction ; Escherichia coli/drug effects ; Escherichia coli/enzymology* ; Escherichia coli/genetics ; Escherichia coli Infections/microbiology ; Humans ; Klebsiella Infections/microbiology ; Klebsiella pneumoniae/drug effects ; Klebsiella pneumoniae/enzymology* ; Klebsiella pneumoniae/genetics ; Korea ; Microbial Sensitivity Tests ; Plasmids* ; beta-Lactamases/analysis* ; beta-Lactamases/genetics
Keywords
AmpC β-lactamase ; Cefoxitin-Hodge test ; Induction test
Abstract
Detection of plasmid-mediated (P-M) AmpC β-lactamase–producing isolates is considered critical for epidemiologic studies and hospital infection control, but the documents of the Clinical and Laboratory Standards Institute do not contain any recommendation for the phenotypic detection. In this study, phenotypic detection methods, cefoxitin–Hodge test and induction test, were evaluated using cefoxitin-resistant Escherichia coli and Klebsiella pneumoniae isolates. The cefoxitin–Hodge test detected all blaCMY-10, and 97.4% of blaCMY-2 allele-positive isolates, but only 57.3% of blaDHA-1 allele-positive isolates. Induction test with an aztreonam and an amoxicillin–clavulanic acid disk was more sensitive than with cefoxitin disk, which detected 86.6% of blaDHA-1 allele-positive isolates. These phenotypic tests should be useful to screen P-M AmpC β-lactamase–producing E. coli and K. pneumoniae isolates.
Full Text
http://www.sciencedirect.com/science/article/pii/S0732889305001951
DOI
10.1016/j.diagmicrobio.2005.07.004
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Chong, Yun Sop(정윤섭)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151501
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