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Identification of four species of the Anopheles hyrcanus complex (Diptera : Culicidae) found in Korea using species-specific primers for Polymerase Chain Reaction Assay

Authors
 Ree Han-Il  ;  Yong Tai-Soon  ;  Hwang Ui-Wook 
Citation
 Medical Entomology and Zoology, Vol.56(3) : 201-205, 2005 
Journal Title
Medical Entomology and Zoology
ISSN
 0424-7086 
Issue Date
2005
MeSH
identification ; Anopheles hyrcanus complex ; PCR ; Korea
Keywords
identification ; Anopheles hyrcanus complex ; PCR ; Korea
Abstract
For identification of four sibling species of the Anopheles hyrcanus complex found in Korea, the 5.8 rDNA-ITS2-28S rDNA region of each species was sequenced and the species-specific primers were designed. The amplified PCR products obtained from each species were analyzed by electrophoresis on 3% agarose gel. The result showed a single species-specific band, i.e. 559bp, 432bp, 322bp and 192bp for An. sinensis, An. sp., An. lesteri and An. pullus, respectively. In conclusion, the species-specific PCR primers designed from ITS2 variable regions functioned successfully and specifically, and can be applied as a useful tool for identifying species of the Hyrcanus complex found in Korea.
Files in This Item:
T200501400.pdf Download
DOI
OAK-2005-06147
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Ree, Han Il(이한일)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151438
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