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P2Y2 agonist induces mucin secretion via Ca2+- and inositol 1,4,5-triphosphate-dependent pathway in human middle ear epithelial cells

DC FieldValueLanguage
dc.contributor.author김정림-
dc.contributor.author김정림-
dc.contributor.author손은진-
dc.contributor.author신지현-
dc.contributor.author윤주헌-
dc.contributor.author최재영-
dc.date.accessioned2017-10-26T06:52:31Z-
dc.date.available2017-10-26T06:52:31Z-
dc.date.issued2005-
dc.identifier.issn0378-5955-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/151433-
dc.description.abstractPurinergic agonists regulate mucin secretion in the airway epithelial cells. This study examined the effects of the apical application of purinergic agonists on Ca(2+) influx ([Ca(2+)](i)), and mucin secretion along with their underlying signaling pathway in normal human middle ear epithelial (NHMEE) cells. The apical membrane of NHMEE cells were stimulated with various purinergic agonists, including UTP, and the [Ca(2+)](i) was measured using a miniature Ussing double perfusion chamber. P2Y(2) receptor in NHMEE cells was also localized by immunohistochemistry. UTP-induced mucin secretion was quantified by an immunoblotting assay. The order of the purinergic agonist potency with respect to [Ca(2+)](i) determined in this study was ATP = UTP > 2-MeSATP > UDP >> adenosine which is consistent with that obtained from P2Y(2) receptor activation. The P2Y(2) receptor is expressed in the apical membrane of monolayered cultured NHMEE cells. Apical UTP-induced [Ca(2+)](i) was inhibited by 2-aminoethoxydiphenyl borate (2-APB) but not by ryanodine. UTP-induced mucin secretion was inhibited by a Ca(2+) chelating agent, BAPTA-AM, and was stimulated by ionomycin. UTP-induced mucin secretion was also suppressed by U73122 and 2-APB, while Calphostin C suppressed it to a small extent and PD98059 was ineffective. Caffeine also inhibited the UTP-induced [Ca(2+)](i) and mucin secretion. These results suggest that the P2Y(2) receptor is expressed in NHMEE cells, and plays a major role in modulating the [Ca(2+)](i) from the IP(3)-sensitive intracellular Ca(2+) store. UTP-induced mucin secretion in NHMEE cells is strongly dependent on Ca(2+)- and IP(3).-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier/North-Holland Biomedical Press.-
dc.relation.isPartOfHEARING RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHMucin-
dc.subject.MESHCaffeine-
dc.subject.MESHSignaling transduction-
dc.subject.MESHIP3-
dc.subject.MESHCa2+-
dc.titleP2Y2 agonist induces mucin secretion via Ca2+- and inositol 1,4,5-triphosphate-dependent pathway in human middle ear epithelial cells-
dc.typeArticle-
dc.publisher.locationNetherlands-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentYonsei Biomedical Research Center (연세의생명연구원)-
dc.contributor.departmentDept. of Otorhinolaryngology (이비인후과학교실)-
dc.contributor.departmentYonsei Biomedical Research Center (연세의생명연구원)-
dc.contributor.departmentDept. of Otorhinolaryngology (이비인후과학교실)-
dc.contributor.departmentDept. of Otorhinolaryngology (이비인후과학교실)-
dc.contributor.googleauthorJae Young Choi-
dc.contributor.googleauthorJi-Hyun Shin-
dc.contributor.googleauthorJung Lim Kim-
dc.contributor.googleauthorSang Ho Jung-
dc.contributor.googleauthorEun Jin Son-
dc.contributor.googleauthorMee Hyun Song-
dc.contributor.googleauthorSung Huhn Kim-
dc.contributor.googleauthorJoo-Heon Yoon-
dc.identifier.doi10.1016/j.heares.2005.05.012-
dc.contributor.localIdA00879-
dc.contributor.localIdA00879-
dc.contributor.localIdA01989-
dc.contributor.localIdA02163-
dc.contributor.localIdA02604-
dc.contributor.localIdA04173-
dc.relation.journalcodeJ00975-
dc.identifier.eissn1878-5891-
dc.identifier.pmid10.1016/j.heares.2005.05.012-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0378595505001917-
dc.subject.keywordMucin-
dc.subject.keywordCaffeine-
dc.subject.keywordSignaling transduction-
dc.subject.keywordIP3-
dc.subject.keywordCa2+-
dc.contributor.alternativeNameKim, Jung Lim-
dc.contributor.alternativeNameKim, Jung Lim-
dc.contributor.alternativeNameSon, Eun Jin-
dc.contributor.alternativeNameShin, Ji Hyun-
dc.contributor.alternativeNameYoon, Joo Heon-
dc.contributor.alternativeNameChoi, Jae Young-
dc.contributor.affiliatedAuthor김정림-
dc.citation.volume209-
dc.citation.number1~2-
dc.citation.startPage24-
dc.citation.endPage31-
dc.identifier.bibliographicCitationHEARING RESEARCH, Vol.209(1~2) : 24-31, 2005-
dc.date.modified2017-05-04-
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers

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