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Adipose tissue engineering using mesenchymal stem cells attached to injectable PLGA spheres

DC FieldValueLanguage
dc.contributor.author박시내-
dc.contributor.author박시내-
dc.contributor.author서활-
dc.contributor.author최유석-
dc.date.accessioned2017-10-26T06:49:55Z-
dc.date.available2017-10-26T06:49:55Z-
dc.date.issued2005-
dc.identifier.issn0142-9612-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/151365-
dc.description.abstractThe reconstruction of soft tissue defects remains a challenge in plastic and reconstructive surgery, and a real clinical need exists for an adequate solution. This study was undertaken in order to differentiate mesenchymal stem cells (MSCs) into adipocytes, and to then assess the possibility of constructing adipose tissue via the attachment of MSCs to injectable PLGA spheres. We also designed injectable PLGA spheres for scar-free transplantation. In this study, MSCs and adipo-MSCs (MSCs cultured in adipogenic medium for 7 days) were attached to PLGA spheres and cultured for 7 days, followed by injection into nude mice for 2 weeks. As a result, the difference between lipid accumulation in adipo-MSCs at 1 and 7 days was much higher in vitro than in the MSCs. Two weeks after injection, a massive amount of new tissue was formed in the APLGA group, whereas only a small amount was formed in the MPLGA group. We verified that the newly formed tissue originated from the injected MSCs via GFP testing, and confirmed that the created tissue was actual adipose tissue by oil red O staining and Western blot (PPARγ and C/EBPα were expressed only in APLGA groups). Therefore, this study presents an efficient model of adipose tissue engineering using MSCs and injectable PLGA spheres.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier Science-
dc.relation.isPartOfBIOMATERIALS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdipocytes/cytology-
dc.subject.MESHAdipose Tissue/pathology*-
dc.subject.MESHAlcian Blue/pharmacology-
dc.subject.MESHAnimals-
dc.subject.MESHAnthraquinones/pharmacology-
dc.subject.MESHAzo Compounds/pharmacology-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCCAAT-Enhancer-Binding Protein-alpha/metabolism-
dc.subject.MESHCell Differentiation-
dc.subject.MESHCell Proliferation-
dc.subject.MESHCell Transplantation-
dc.subject.MESHCicatrix-
dc.subject.MESHGreen Fluorescent Proteins/chemistry-
dc.subject.MESHGreen Fluorescent Proteins/metabolism-
dc.subject.MESHHexosaminidases/metabolism-
dc.subject.MESHLactic Acid/chemistry*-
dc.subject.MESHLipid Metabolism-
dc.subject.MESHMesenchymal Stem Cells/cytology*-
dc.subject.MESHMice-
dc.subject.MESHMice, Nude-
dc.subject.MESHMicroscopy, Electron, Scanning-
dc.subject.MESHMicroscopy, Fluorescence-
dc.subject.MESHMicrospheres*-
dc.subject.MESHPolyglycolic Acid/chemistry*-
dc.subject.MESHPolylactic Acid-Polyglycolic Acid Copolymer-
dc.subject.MESHPolymers/chemistry*-
dc.subject.MESHRabbits-
dc.subject.MESHTime Factors-
dc.subject.MESHTissue Engineering/methods*-
dc.subject.MESHTransfection-
dc.titleAdipose tissue engineering using mesenchymal stem cells attached to injectable PLGA spheres-
dc.typeArticle-
dc.publisher.locationNetherlands-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Medical Engineering (의학공학교실)-
dc.contributor.departmentDept. of Medical Engineering (의학공학교실)-
dc.contributor.departmentDept. of Medical Engineering (의학공학교실)-
dc.contributor.googleauthorYu Suk Choi-
dc.contributor.googleauthorSi-Nae Park-
dc.contributor.googleauthorHwal Suh-
dc.identifier.doi10.1016/j.biomaterials.2005.02.022-
dc.contributor.localIdA01552-
dc.contributor.localIdA01552-
dc.contributor.localIdA01924-
dc.contributor.localIdA04134-
dc.relation.journalcodeJ00312-
dc.identifier.eissn1878-5905-
dc.identifier.pmid15949551-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0142961205001882-
dc.subject.keywordAdipose tissue engineering-
dc.subject.keywordPLGA-
dc.subject.keywordMesenchymal stem cell-
dc.subject.keywordInjectable scaffold-
dc.contributor.alternativeNamePark, Si Nae-
dc.contributor.alternativeNamePark, Si Nae-
dc.contributor.alternativeNameSuh, Hwal-
dc.contributor.alternativeNameChoi, Yu Suk-
dc.contributor.affiliatedAuthor박시내-
dc.citation.volume26-
dc.citation.number29-
dc.citation.startPage5855-
dc.citation.endPage5863-
dc.identifier.bibliographicCitationBIOMATERIALS, Vol.26(29) : 5855-5863, 2005-
dc.date.modified2017-05-04-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers

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