Cited 17 times in
Isogenic variation of Helicobacter pylori strain resulting in heteroresistant antibacterial phenotypes in a single host in vivo
DC Field | Value | Language |
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dc.contributor.author | 이용찬 | - |
dc.contributor.author | 표재희 | - |
dc.date.accessioned | 2017-10-26T06:46:20Z | - |
dc.date.available | 2017-10-26T06:46:20Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1083-4389 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/151272 | - |
dc.description.abstract | BACKGROUND: Antibiotic-susceptible and -resistant Helicobacter pylori can be present simultaneously in the same host. The aim of this study was to evaluate the genomic diversity of H. pylori strains resulting in heteroresistant antibacterial phenotypes. MATERIALS AND METHODS: Twenty-one pairs of H. pylori strains isolated from the antrum and body displaying heteroresistant antibacterial phenotypes were included. We compared the genotypes of paired-isolates by random arbitrarily primed polymerase chain reaction (PCR), flagella gene PCR-based restriction fragment length polymorphism, and flaA gene sequencing. In metronidazole-heteroresistant isolates, the sequence variation of rdxA and frxA genes was analyzed using phylogenetic analysis. RESULTS: The DNA fingerprinting patterns of the paired isolates revealed that 12 pairs (57.1%) were identical, whereas one pair (3.8%) was different. The remaining eight pairs (38.1%) of isolates showed minor heterogenecity in fingerprinting patterns. In flaA gene sequencing, these identical and similar isolates showed close sequence similarity between the antrum and body, whereas different isolate showed 31 points of different nucleotide sequences. Phylogenetic analysis of the metronidazole-heteroresistant pairs showed consistent genetic relatedness of each paired isolates despite the sequence variation of the rdxA or frxA genes in five pairs (71.4%). CONCLUSIONS: These results suggest that continuing genomic diversities in the same strain may play an important role in modulating the antibiotic-heteroresistant H. pylori in vivo. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Wiley-Blackwell | - |
dc.relation.isPartOf | HELICOBACTER | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Anti-Bacterial Agents/pharmacology | - |
dc.subject.MESH | Bacterial Proteins/genetics | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | DNA Fingerprinting | - |
dc.subject.MESH | Drug Resistance, Bacterial* | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Genetic Variation* | - |
dc.subject.MESH | Genotype | - |
dc.subject.MESH | Helicobacter Infections/microbiology* | - |
dc.subject.MESH | Helicobacter pylori/classification* | - |
dc.subject.MESH | Helicobacter pylori/drug effects | - |
dc.subject.MESH | Helicobacter pylori/genetics* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Metronidazole/pharmacology | - |
dc.subject.MESH | Microbial Sensitivity Tests | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Phenotype | - |
dc.subject.MESH | Phylogeny | - |
dc.subject.MESH | Polymerase Chain Reaction | - |
dc.subject.MESH | Polymorphism, Restriction Fragment Length | - |
dc.title | Isogenic variation of Helicobacter pylori strain resulting in heteroresistant antibacterial phenotypes in a single host in vivo | - |
dc.type | Article | - |
dc.publisher.location | England | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.googleauthor | Yong Chan Lee | - |
dc.contributor.googleauthor | Sun-Young Lee | - |
dc.contributor.googleauthor | Jae Hee Pyo | - |
dc.contributor.googleauthor | Dong H. Kwon | - |
dc.contributor.googleauthor | Jong Chul Rhee | - |
dc.contributor.googleauthor | Jae J. Kim | - |
dc.identifier.doi | 10.1111/j.1523-5378.2005.00316.x | - |
dc.contributor.localId | A02988 | - |
dc.contributor.localId | A04243 | - |
dc.relation.journalcode | J00981 | - |
dc.identifier.eissn | 1523-5378 | - |
dc.identifier.pmid | 15904482 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/j.1523-5378.2005.00316.x/abstract | - |
dc.subject.keyword | Helicobacter pylori | - |
dc.subject.keyword | drug resistance | - |
dc.subject.keyword | DNA fingerprinting | - |
dc.contributor.alternativeName | Lee, Yong Chan | - |
dc.contributor.alternativeName | Lee, Yong Chan | - |
dc.contributor.alternativeName | Pyo, Jae Hee | - |
dc.contributor.affiliatedAuthor | 이용찬 | - |
dc.citation.volume | 10 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 240 | - |
dc.citation.endPage | 248 | - |
dc.identifier.bibliographicCitation | HELICOBACTER, Vol.10(3) : 240-248, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 43993 | - |
dc.type.rims | ART | - |
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