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Characterization of newly established oral cancer cell lines derived from six squamous cell carcinoma and two mucoepidermoid carcinoma cells

Authors
 Eun Ju Lee  ;  Jin Kim  ;  Seoung Ae Lee  ;  Eun-Jung Kim  ;  Yong-Chan Chun  ;  Mi Heon Ryu  ;  Jong-In Yook 
Citation
 EXPERIMENTAL AND MOLECULAR MEDICINE, Vol.37(5) : 379-390, 2005 
Journal Title
 EXPERIMENTAL AND MOLECULAR MEDICINE 
ISSN
 1226-3613 
Issue Date
2005
MeSH
Adult ; Aged ; Animals ; Base Sequence ; Biomarkers, Tumor ; Carcinoma, Mucoepidermoid/genetics ; Carcinoma, Mucoepidermoid/metabolism* ; Carcinoma, Mucoepidermoid/pathology* ; Carcinoma, Squamous Cell/genetics ; Carcinoma, Squamous Cell/metabolism* ; Carcinoma, Squamous Cell/pathology* ; Cell Line, Tumor* ; Epithelial Cells/metabolism ; ErbB Receptors/genetics ; ErbB Receptors/metabolism ; Female ; Humans ; Male ; Mice ; Mice, Nude ; Middle Aged ; Mouth Neoplasms/genetics ; Mouth Neoplasms/metabolism* ; Mouth Neoplasms/pathology* ; Mutation/genetics ; Papillomaviridae/physiology ; Tumor Suppressor Protein p53/genetics ; Tumor Suppressor Protein p53/metabolism ; Xenograft Model Antitumor Assays
Keywords
16264262
Abstract
Since genetic abnormalities of human cancer are greatly geographically dependent, cultural and environmental backgrounds are thought to be closely related to the carcinogenic process. In the present study, eight human cell lines were established by culture from untreated carcinomas of the oral cancer, of which five were from primary oral squamous cell carcinomas (OSC), one from a mucoepidermoid carcinoma (MEC) and one each originating from metastatic OSC and MEC. All the studied tumor lines grew as monolayers, and showed: i) an epithelial origin by the presence of cytokeratin, and ii) tumorigenic potential in nude mice. Western blot analysis revealed i) over expression of EGFR in six of the cell lines ii) decreased expression of E- cadherin in six cell lines compared to normal human oral mucosa. A mutational analysis showed: point mutations of p53 at exon 7, with transversion, and at exon 8, with transition. These well-characterized human YD cell lines should serve as useful tools in the study of the molecular pathogenesis and biological characteristics of head and neck cancer cells, and in the future testing of new therapeutic reagents for oral cancer.
Files in This Item:
T200500871.pdf Download
DOI
10.1038/emm.2005.48
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jin(김진)
Yook, Jong In(육종인) ORCID logo https://orcid.org/0000-0002-7318-6112
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151056
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