Cited 38 times in
Induction of proinflammatory mediators requires activation of the TRAF, NIK, IKK and NF-κB signal transduction pathway in astrocytes infected with Escherichia coli
DC Field | Value | Language |
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dc.contributor.author | 최인홍 | - |
dc.date.accessioned | 2017-10-26T06:30:53Z | - |
dc.date.available | 2017-10-26T06:30:53Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0009-9104 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/150908 | - |
dc.description.abstract | Escherichia coli is associated with inflammation in the brain. To investigate whether astrocytes are involved in E. coil-induced inflammation, we assessed the levels of expression of proinflammatory mediators produced by E. coli-infected astrocytes. E. coli infection in primary human astrocytes and cell lines increased expression of the CXC chemokine IL-8/GRO-α, the CC chemokine MCP-1, TNF-α, and iNOS. E. coli infection activated p65/p50 heterodimeric NF-κB and concurrently decreased the signals of IκBα. Blocking the NF-κB signals by IκBα-superrepressor-containing retrovirus or antisense p50 oligonucleotide transfection resulted in down-regulation of expression of the proinflammatory mediators. Furthermore, superrepressors of IκBα, IκB kinase (IKK) or NF-κB inducing kinase (NIK) inhibited the up-regulated expression of the downstream target genes of NF-κB such as IL-8 and MCP-1, and superrepressors of TNF receptor-associated factor (TRAF)2 and TRAF5 also inhibited expression of the E. coli-induced target genes of NF-κB. These results indicate that proinflammatory mediators such as the CXC chemokine IL-8/GRO-α, the CC chemokine MCP-1, TNF-α, and iNOS can be expressed in E. coli-infected astrocytes via an NF-κB pathway, suggesting that these mediators may contribute to inflammation in the brain, including infiltration of inflammatory cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Blackwell Scientific Publications | - |
dc.relation.isPartOf | CLINICAL AND EXPERIMENTAL IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Astrocytes/immunology* | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Chemokine CCL2/immunology | - |
dc.subject.MESH | Chemokine CXCL1 | - |
dc.subject.MESH | Chemokines/immunology* | - |
dc.subject.MESH | Chemokines, CXC/immunology | - |
dc.subject.MESH | Escherichia coli Infections/immunology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | I-kappa B Kinase | - |
dc.subject.MESH | Intercellular Signaling Peptides and Proteins/immunology | - |
dc.subject.MESH | Interleukin-8/immunology | - |
dc.subject.MESH | NF-kappa B/immunology | - |
dc.subject.MESH | Nitric Oxide Synthase/immunology | - |
dc.subject.MESH | Nitric Oxide Synthase Type II | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Protein-Serine-Threonine Kinases/immunology | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction/methods | - |
dc.subject.MESH | Signal Transduction/immunology* | - |
dc.subject.MESH | TNF Receptor-Associated Factor 2/immunology | - |
dc.subject.MESH | TNF Receptor-Associated Factor 5/immunology | - |
dc.subject.MESH | Tumor Necrosis Factor-alpha/immunology | - |
dc.title | Induction of proinflammatory mediators requires activation of the TRAF, NIK, IKK and NF-κB signal transduction pathway in astrocytes infected with Escherichia coli | - |
dc.type | Article | - |
dc.publisher.location | England | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학교실) | - |
dc.contributor.googleauthor | J. M. Kim | - |
dc.contributor.googleauthor | Y.-K. Oh | - |
dc.contributor.googleauthor | J. H. Lee | - |
dc.contributor.googleauthor | D. Y. Im | - |
dc.contributor.googleauthor | Y.-J. Kim | - |
dc.contributor.googleauthor | J. Youn | - |
dc.contributor.googleauthor | C.-H. Lee | - |
dc.contributor.googleauthor | H. Son | - |
dc.contributor.googleauthor | Y.-S. Lee | - |
dc.contributor.googleauthor | J. Y. Park | - |
dc.contributor.googleauthor | I.-H. Choi | - |
dc.identifier.doi | 10.1111/j.1365-2249.2005.02804.x | - |
dc.contributor.localId | A04167 | - |
dc.relation.journalcode | J00550 | - |
dc.identifier.eissn | 1365-2249 | - |
dc.identifier.pmid | 15932506 | - |
dc.subject.keyword | astrocytes | - |
dc.subject.keyword | Escherichia coli | - |
dc.subject.keyword | NF‐κB | - |
dc.subject.keyword | proinflammatory mediators | - |
dc.contributor.alternativeName | Choi, In Hong | - |
dc.citation.volume | 140 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 450 | - |
dc.citation.endPage | 460 | - |
dc.identifier.bibliographicCitation | CLINICAL AND EXPERIMENTAL IMMUNOLOGY, Vol.140(3) : 450-460, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 42758 | - |
dc.type.rims | ART | - |
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