Cited 26 times in
P-Cadherin is decreased in diabetic glomeruli and in glucose-stimulated podocytes in vivo and in vitro studies
DC Field | Value | Language |
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dc.contributor.author | 강신욱 | - |
dc.contributor.author | 김주성 | - |
dc.contributor.author | 김진주 | - |
dc.contributor.author | 류동열 | - |
dc.contributor.author | 유태현 | - |
dc.contributor.author | 정동섭 | - |
dc.contributor.author | 최훈영 | - |
dc.contributor.author | 한대석 | - |
dc.contributor.author | 허종호 | - |
dc.date.accessioned | 2017-10-26T06:22:55Z | - |
dc.date.available | 2017-10-26T06:22:55Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0931-0509 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/150854 | - |
dc.description.abstract | BACKGROUND: Proteinuria is a cardinal feature of glomerular disease, including diabetic nephropathy, and the glomerular filtration barrier acts as a filter, restricting protein excretion in urine. We tested whether the expression of P-cadherin, a molecule known to be located at the slit diaphragm, was altered by diabetes in vivo and by high glucose in vitro. METHODS: In vivo, 24 Sprague-Dawley rats were injected with diluent [control (C), n=8] or streptozotocin intraperitoneally and the latter were left untreated (DM, n=8) or treated with insulin (DM+I, n=8) for 6 weeks. In vitro, immortalized mouse podocytes were cultured in media with 5.6 mM glucose (LG), LG+19.4 mM mannitol (LG+M) or 25 mM glucose (HG) with or without protein kinase C (PKC) inhibitor (10(-7) M calphostin C or 10(-6) M GF 109203X). Reverse transcription-polymerase chain reaction, western blotting for P-cadherin mRNA and protein expression, respectively, were performed with sieved glomeruli and cell lysates, and immunofluorescence staining was undertaken with renal tissue. RESULTS: Twenty-four hour urinary albumin excretion was significantly higher in DM compared with C and DM+I rats (P<0.05). Glomerular P-cadherin mRNA expression was significantly lower in DM (1.36+/-0.20x10(-2) attm/ng RNA) than in C rats (2.61+/-0.33x10(-2) attm/ng RNA) (P<0.05). P-Cadherin protein expression, assessed by western blot and immunofluorescence staining, was also decreased in DM compared with C and DM+I glomeruli. HG significantly reduced P-cadherin mRNA and protein expression in cultured podocytes by 42% and 62%, respectively (P<0.05), and these decrements were ameliorated by PKC inhibitor. CONCLUSIONS: Diabetes in vivo and exposure of podocytes to HG in vitro reduced P-cadherin mRNA and protein expression, and PKC was involved in the regulation of HG-induced down-regulation of P-cadherin. These findings suggest that the decrease in P-cadherin expression is connected with the early changes of diabetic nephropathy and, thus, may contribute to the development of proteinuria. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Oxford University Press | - |
dc.relation.isPartOf | NEPHROLOGY DIALYSIS TRANSPLANTATION | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | P-cadherin | - |
dc.subject.MESH | diabetic nephropathy | - |
dc.subject.MESH | high glucose | - |
dc.subject.MESH | podocyte | - |
dc.subject.MESH | proteinuria | - |
dc.title | P-Cadherin is decreased in diabetic glomeruli and in glucose-stimulated podocytes in vivo and in vitro studies | - |
dc.type | Article | - |
dc.publisher.location | England | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.googleauthor | Zhong-Gao Xu | - |
dc.contributor.googleauthor | Dong-Ryeol Ryu | - |
dc.contributor.googleauthor | Tae-Hyun Yoo | - |
dc.contributor.googleauthor | Dong-Sub Jung | - |
dc.contributor.googleauthor | Jin-Ju Kim | - |
dc.contributor.googleauthor | Hyung-Jong Kim | - |
dc.contributor.googleauthor | Hoon-Young Choi | - |
dc.contributor.googleauthor | Joo-Seong Kim | - |
dc.contributor.googleauthor | Sharon G. Adler | - |
dc.contributor.googleauthor | Rama Natarajan | - |
dc.contributor.googleauthor | Dae-Suk Han | - |
dc.contributor.googleauthor | Shin-Wook Kang | - |
dc.identifier.doi | 10.1093/ndt/gfh642 | - |
dc.contributor.localId | A00053 | - |
dc.contributor.localId | A00934 | - |
dc.contributor.localId | A01029 | - |
dc.contributor.localId | A01323 | - |
dc.contributor.localId | A02526 | - |
dc.contributor.localId | A03594 | - |
dc.contributor.localId | A04226 | - |
dc.contributor.localId | A04272 | - |
dc.contributor.localId | A04366 | - |
dc.relation.journalcode | J02316 | - |
dc.identifier.eissn | 1460-2385 | - |
dc.identifier.pmid | 10.1093/ndt/gfh642 | - |
dc.subject.keyword | P-cadherin | - |
dc.subject.keyword | diabetic nephropathy | - |
dc.subject.keyword | high glucose | - |
dc.subject.keyword | podocyte | - |
dc.subject.keyword | proteinuria | - |
dc.contributor.alternativeName | Kang, Shin Wook | - |
dc.contributor.alternativeName | Kim, Joo Sung | - |
dc.contributor.alternativeName | Kim, Jin Ju | - |
dc.contributor.alternativeName | Ryu, Dong Ryeol | - |
dc.contributor.alternativeName | Yoo, Tae Hyun | - |
dc.contributor.alternativeName | Jung, Dong Sub | - |
dc.contributor.alternativeName | Choi, Hoon Young | - |
dc.contributor.alternativeName | Han, Dae Suk | - |
dc.contributor.alternativeName | Xu, Zhong Gao | - |
dc.citation.volume | 20 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 524 | - |
dc.citation.endPage | 531 | - |
dc.identifier.bibliographicCitation | NEPHROLOGY DIALYSIS TRANSPLANTATION, Vol.20(3) : 524-531, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 44765 | - |
dc.type.rims | ART | - |
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