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A Feasibility Study of Adenosine Triphosphate-based Chemotherapy Response Assay (ATP-CRA) as a Chemosensitivity Test for Lung Cancer

 Shin Myung Kang  ;  Moo Suk Park  ;  Joon Chang  ;  Se Kyu Kim  ;  Haeryoung Kim  ;  Dong-Hwan Shin  ;  Kyung Young Chung  ;  Dae Joon Kim  ;  Joo Hyuk Sohn  ;  Sung Ho Choi  ;  Jeongmi Kim  ;  Eun Jin Yoon  ;  Joo-Hang Kim 
 Cancer Research and Treatment, Vol.37(4) : 223-227, 2005 
Journal Title
 Cancer Research and Treatment 
Issue Date
PURPOSE: A chemosensitivity test can reflect the differences in responses of individual cancer patients to chemotherapeutic agents. The adenosine triphosphate-based chemotherapy response assay (ATP-CRA) is an accurate method, which does not require a large amount of tissue specimen. So far, no studies have evaluated the utility of the ATP-CRA in Korea. Therefore, we investigated the clinical usefulness of the ATP-CRA in 53 patients with lung cancer. MATERIALS AND METHODS: Tumor tissues were obtained from bronchoscopic biopsies or surgical resections. The validity of ATP-CRA was assessed focusing on the success rate, experimental error level (intraassay mean coefficient of variation [CV]) and reproducibility. RESULTS: The overall success rate of ATP-CRA was 90.6% (48/53). Normal cells were effectively eliminated from the tumor tissues with the use of ficoll gradient centrifugation and immunomagnetic separation, which was confirmed using loss of heterozygosity analysis of the 3p deletion. The mean CV of ATP assays was 10.5+/-4.6%. The reproducibility of ATP assays was 94+/-3.8%. The results of the ATP assays were reported to physicians within 7 days of specimen collection. More than 6 anticancer drugs were tested on the tumor specimens obtained from bronchoscopic biopsies. CONCLUSION: The ATP-CRA is a stable, accurate and potentially practical chemosensitivity test in patients with lung cancer.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
강신명(Kang, Shin Myung)
김대준(Kim, Dae Joon)
김세규(Kim, Se Kyu)
김주항(Kim, Joo Hang)
김혜령(Kim, Hae Ryoung)
박무석(Park, Moo Suk) ORCID logo https://orcid.org/0000-0003-0820-7615
손주혁(Sohn, Joo Hyuk) ORCID logo https://orcid.org/0000-0002-2303-2764
신동환(Shin, Dong Hwan)
장준(Chang, Joon) ORCID logo https://orcid.org/0000-0003-4542-6841
정경영(Chung, Kyung Young)
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