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NAG-1 expression and apoptosis induction in endometrial stromal cells of patients with endometriosis by histone deacetylase inhibitor trichostatin A

Other Titles
 자궁내막증 환자의 자궁내막세포에서 trichostatin A에 의한 NAG-1의 발현과 세포자멸사의 변화 
 Dept. of Obstetrics and Gynecology (산부인과학교실) 
Issue Date
Objective The present study investigated the effects of trichostatin A (TSA) on non-steroidal anti-inflammatory drug-activated gene-1 (NAG-1) expression and apoptosis in human endometrial stromal cells (HESCs) and assessed whether these effects are enhanced in the presence of 5-aza-2''-deoxycytidine (5-aza-dC). Methods Eutopic endometrial tissue samples were obtained from 10 patients with fibroids who underwent hysterectomy. Ectopic endometrial tissues were obtained from 15 patients with endometriotic cysts who underwent cystectomy. HESCs were isolated and cultured with different concentrations of TSA. Real-time PCR and western blotting were used to evaluate NAG-1 mRNA and protein levels, respectively. Apoptosis was assessed by flow cytometry. Results TSA induced NAG-1 expression and apoptosis in HESCs. Gene knockdown experiments using small interfering RNA confirmed an association between NAG-1 expression and TSA-induced apoptosis. We did not observe synergy in the apoptosis-inducing effects of TSA and 5-aza-dC in HESCs. Conclusion TSA induced apoptosis in HESCs via induction of NAG-1 expression. These results suggest that TSA-and possibly other HDACIs or agents that induce NAG-1 expression-can be effective for treating endometriosis.
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1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 3. Dissertation
Yonsei Authors
Seo, Seok Kyo(서석교) ORCID logo https://orcid.org/0000-0003-3404-0484
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