Identification and clinical implications of circulating microRNAs for estrogen receptor positive breast cancer
Other Titles
에스트로겐 수용체 양성인 유방암에 특이적인 혈중 microRNA 의 발견 및 임상적 의의 검증
Authors
박인혜
Department
Dept. of Internal Medicine (내과학교실)
Issue Date
2016
Description
의학과/박사
Abstract
Background
MicroRNA expression is frequently dysregulated in cancer and it could be used potentially as a disease classifier and a prognostic tool in cancer. It has been reported that the cancer associated specific microRNAs were detected in blood stably. The objective of this study was to discover a panel of circulating microRNAs as potential ER+/HER2- breast cancer biomarkers.
Methods
We compared the levels of circulating microRNAs in blood samples from 11 ER+/HER2- advanced breast cancer patients with age-matched 5 control subjects by using microarray-based expression profiling followed by real-time quantitative polymerase cycle reaction (RT-qPCR) validation. We evaluated the association between the levels of microRNA and clinical outcomes of ER+/HER2- metastatic breast cancer.
Results
We found that 4 microRNAs (miR-1280, miR-1260, miR-1274b, miR-720) were up-regulated in blood from breast cancer patients (P<0.05, constrained by at least two-fold expression change). We evaluated the level of each microRNAs in 40 control subjects, 180 early breast cancer patients (EBC), and 52 metastatic breast cancer patients (MBC). Among those, the levels of miR-1280 significantly increased in breast cancer patients and those were correlated with tumor status (control<
Conclusions
These findings suggest that a circulating tRNA derived miRNA, miR-1280 is differently expressed in breast cancer patients and it can be used as a potential biomarker for ER positive breast cancer.