Cited 0 times in

Anti-cancer effect of luminacin, a marine microbial extract, in head and neck squamous cell carcinoma progression via autophagic cell death

Other Titles
 신규 해양미생물 추출물, luminacin의 autophagic cell death 를 통한 두경부암 항암효과분석 
Authors
 신유섭 
Department
 Dept. of Otorhinolaryngology (이비인후과학교실) 
Issue Date
2015
Description
의과대학/박사
Abstract
To determine whether Luminacin, a marine microbial extract from the marine Streptomyces species, has anti-tumor effects on head and neck squamous cell carcinoma (HNSCC) cell lines via autophagic cell death. The inhibition of cell survival and increased cell death was measured using cell viability, colony forming, and apoptosis assays. Migration and invasion abilities of head and cancer cells were evaluated using wound healing, scattering, and invasion assays. Changes in the signal pathway related to autophagic cell death were investigated. Drug toxicity of Luminacin was examined in in vitro the HaCaT cells and an in vivo zebrafish model. Luminacin showed potent cytotoxicity in HNSCC cells in cell viability, colony forming and FACS analysis. In vitro migration and invasion of HNSCC cells were attenuated by Luminacin treatment. Combined with Beclin-1 and LC3B, Luminacin induced autophagic cell death in head and neck cancer cells. In addition, in a zebrafish model used for toxicity testing, embryonic or neural toxicity was not resulted in by Luminacin treatment with a cytotoxic concentration for HNSCC cells. Taken together, these results demonstrate that Luminacin induces the inhibition of growth and cancer progression via autophagic cell death in HNSCC cell lines, indicating a possible alternative chemotherapeutic approach for the treatment of HNSCC.
Files in This Item:
T013643.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 3. Dissertation
Yonsei Authors
Shin, Yoo Seob(신유섭)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/148687
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links