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Catabolic role of a three-component salicylate oxygenase from Sphingomonas yanoikuyae B1 in polycyclic aromatic hydrocarbon degradation

DC Field Value Language
dc.contributor.author이준행-
dc.date.accessioned2017-05-04T07:40:19Z-
dc.date.available2017-05-04T07:40:19Z-
dc.date.issued2005-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/147627-
dc.description.abstractSphingomonas yanoikuyae B1 possesses several different multicomponent oxygenases involved in metabolizing aromatic compounds. Six different pairs of genes encoding large and small subunits of oxygenase iron-sulfur protein components have previously been identified in a gene cluster involved in the degradation of both monocyclic and polycyclic aromatic hydrocarbons. Insertional inactivation of one of the oxygenase large subunit genes, bphA1c, results in a mutant strain unable to grow on naphthalene, phenanthrene, or salicylate. The knockout mutant accumulates salicylate from naphthalene and 1-hydroxy-2-naphthoic acid from phenanthrene indicating the loss of salicylate oxygenase activity. Complementation experiments verify that the salicylate oxygenase in S. yanoikuyae B1 is a three-component enzyme consisting of an oxygenase encoded by bphA2cA1c, a ferredoxin encoded by the adjacent bphA3, and a ferredoxin reductase encoded by bphA4 located over 25kb away. Expression of bphA3-bphA2c-bphA1c genes in Escherichia coli demonstrated the ability of salicylate oxygenase to convert salicylate to catechol and 3-, 4-, and 5-methylsalicylate to methylcatechols.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier-
dc.relation.isPartOfBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBiodegradation, Environmental-
dc.subject.MESHCatechols/metabolism-
dc.subject.MESHCloning, Molecular-
dc.subject.MESHEscherichia coli/enzymology-
dc.subject.MESHEscherichia coli/genetics-
dc.subject.MESHFerredoxin-NADP Reductase/genetics-
dc.subject.MESHFerredoxin-NADP Reductase/metabolism-
dc.subject.MESHFerredoxins/genetics-
dc.subject.MESHFerredoxins/metabolism-
dc.subject.MESHHydrocarbons, Aromatic/chemistry-
dc.subject.MESHHydrocarbons, Aromatic/metabolism-
dc.subject.MESHMultigene Family-
dc.subject.MESHNaphthalenes/metabolism-
dc.subject.MESHOxygenases/genetics-
dc.subject.MESHOxygenases/metabolism*-
dc.subject.MESHPhenanthrenes/metabolism-
dc.subject.MESHPolycyclic Aromatic Hydrocarbons/chemistry-
dc.subject.MESHPolycyclic Aromatic Hydrocarbons/metabolism*-
dc.subject.MESHSalicylates/metabolism-
dc.subject.MESHSphingomonas/chemistry-
dc.subject.MESHSphingomonas/enzymology*-
dc.subject.MESHSphingomonas/genetics-
dc.subject.MESHSphingomonas/metabolism-
dc.subject.MESHTime Factors-
dc.titleCatabolic role of a three-component salicylate oxygenase from Sphingomonas yanoikuyae B1 in polycyclic aromatic hydrocarbon degradation-
dc.typeArticle-
dc.publisher.locationUnited States-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Ophthalmology (안과학교실)-
dc.contributor.googleauthorOkyoung Cho-
dc.contributor.googleauthorKi Young Choi-
dc.contributor.googleauthorGerben J. Zylstra-
dc.contributor.googleauthorYoung-Soo Kim-
dc.contributor.googleauthorSeong-Ki Kim-
dc.contributor.googleauthorJoon H. Lee-
dc.contributor.googleauthorHo-Yong Sohn-
dc.contributor.googleauthorGi-Seok Kwon-
dc.contributor.googleauthorYoung Min Kim-
dc.contributor.googleauthorEungbin Kim-
dc.identifier.doi10.1016/j.bbrc.2004.12.060-
dc.contributor.localIdA03180-
dc.relation.journalcodeJ00281-
dc.identifier.eissn1090-2104-
dc.identifier.pmid15649397-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006291X04028591-
dc.subject.keywordSphingomonas-
dc.subject.keywordSalicylate oxygenase-
dc.subject.keywordPhenanthrene-
dc.subject.keywordNaphthalene-
dc.subject.keywordBiphenyl-
dc.subject.keywordXylene-
dc.contributor.alternativeNameLee, Joon Haeng-
dc.citation.volume327-
dc.citation.number3-
dc.citation.startPage656-
dc.citation.endPage662-
dc.identifier.bibliographicCitationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.327(3) : 656-662, 2005-
dc.date.modified2017-05-04-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers

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