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3-nitropropionic Acid 투여 후 마우스 선조체에서 Apurinic/Apyrimidinic Endonuclease의 발현과 신경세포고사
DC Field | Value | Language |
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dc.contributor.author | 이병인 | - |
dc.contributor.author | 조경주 | - |
dc.date.accessioned | 2017-05-04T07:39:47Z | - |
dc.date.available | 2017-05-04T07:39:47Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1738-1428 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/147612 | - |
dc.description.abstract | BACKGROUND: 3-Nitroporpionic acid (3-NP) is an irreVersible inhibitor of succinate dehydrogenase in mitochondria and can induce apoptosis-like cell death in the striatum. It has been reported that oxidative stress plays a role in the 3-NP induced neuronal damage. 3-NP induced striatal damage is implicated in the pathogenesis of several neurological diseases, such as chronic neurodegenerative diseases and stroke. The DNA repair enzyme, apurinic/apyrimidinic endonuclease (APE), is a multifunctional protein in the DNA base excision repair (BER) pathway. To clarify the relationship between APE and neuronal cell death associated with the apoptosis in the striatum was induced by 3-NP in vivo. METHODS: After intra-striatal injection of 3-NP, expression of the APE protein and mRNA were evaluated by Western blot, immunohistochemistry, RT-PCR and DNA fragmentation patterns. Oxidative DNA damage was investigated by detection of oxidized DNA, AP site and superoxide. RESULTS: Expression levels of APE was rapidly reduced as early as 1hr after injection of 3-NP. DNA fragmentation was observed 24 hours after 3-NP treatment but not 4 hours. APE gene expression was increased to 1hr after 3-NP treatment. The number of AP sites were reduced and the reduction of APE proteins were blocked by a superoxide scavenger, MnTBAP-treatment. CONCLUSIONS: These results suggest that the reduction of APE is the preceding event of DNA fragmentation that causes apoptosis and a decrease of APE may be induced by ROS after 3-NP treatment. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 510~518 | - |
dc.language | Korean | - |
dc.publisher | Korean Neurological Association | - |
dc.relation.isPartOf | Journal of the Korean Neurological Association | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | 3-Nitropropionic acid | - |
dc.subject.MESH | Apurinic | - |
dc.subject.MESH | apyrimidinic endonuclese | - |
dc.subject.MESH | Reactive oxygen species | - |
dc.subject.MESH | Mananese tetrakis (40benzoic acid porphyrin) | - |
dc.subject.MESH | Reverse transcription polymerase chain reaction | - |
dc.title | 3-nitropropionic Acid 투여 후 마우스 선조체에서 Apurinic/Apyrimidinic Endonuclease의 발현과 신경세포고사 | - |
dc.title.alternative | Expression of Apurinic/apyrimidinic Endonuclease and Neuronal Apoptosis in the Striatum after Treatment of 3-Nitropropionic Acid in Mice | - |
dc.type | Article | - |
dc.publisher.location | Korea | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Neurology (신경과학교실) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.googleauthor | 조경주 | - |
dc.contributor.googleauthor | 이두재 | - |
dc.contributor.googleauthor | 이병인 | - |
dc.contributor.googleauthor | 김경환 | - |
dc.identifier.doi | OAK-2005-03351 | - |
dc.contributor.localId | A02797 | - |
dc.contributor.localId | A03804 | - |
dc.relation.journalcode | J01835 | - |
dc.relation.journalsince | 2004~ | - |
dc.relation.journalbefore | ~2003 Journal of the Korean Neurological Association (대한신경과학회지) | - |
dc.subject.keyword | 3-Nitropropionic acid | - |
dc.subject.keyword | Apurinic | - |
dc.subject.keyword | apyrimidinic endonuclese | - |
dc.subject.keyword | Reactive oxygen species | - |
dc.subject.keyword | Mananese tetrakis (40benzoic acid porphyrin) | - |
dc.subject.keyword | Reverse transcription polymerase chain reaction | - |
dc.contributor.alternativeName | Lee, Byung In | - |
dc.contributor.alternativeName | Cho, Kyuong Joo | - |
dc.citation.volume | 23 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 510 | - |
dc.citation.endPage | 518 | - |
dc.identifier.bibliographicCitation | Journal of the Korean Neurological Association, Vol.23(4) : 510-518, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 40384 | - |
dc.type.rims | ART | - |
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