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Effects of Glial Transplantation on Functional Recovery following Acute Spinal Cord Injury

 Kyung Hee Lee  ;  Do Heum Yoon  ;  Yong Gou Park  ;  Bae Hwan Lee 
 JOURNAL OF NEUROTRAUMA, Vol.22(5) : 575-589, 2005 
Journal Title
Issue Date
2',3'-Cyclic-Nucleotide Phosphodiesterases/metabolism ; Animals ; Animals, Newborn ; Astrocytes/metabolism ; Brain Stem/cytology ; Brain Stem/physiology ; Bromodeoxyuridine ; Cell Differentiation/physiology ; Cell Proliferation ; Cells, Cultured ; Disease Models, Animal ; Efferent Pathways/cytology ; Efferent Pathways/physiology ; Fluorescent Dyes ; Graft Survival/physiology ; Male ; Nerve Regeneration/physiology* ; Neuroglia/cytology ; Neuroglia/metabolism* ; Neuroglia/transplantation* ; Neuronal Plasticity/physiology ; Oligodendroglia/metabolism ; Rats ; Rats, Sprague-Dawley ; Recovery of Function/physiology* ; Spinal Cord Injuries/pathology ; Spinal Cord Injuries/physiopathology ; Spinal Cord Injuries/therapy* ; Stem Cell Transplantation/methods* ; Stem Cells/cytology ; Stem Cells/metabolism* ; Treatment Outcome
Numerous efforts have been made to maximize the efficacy of treatment for spinal cord injury (SCI). Recently, oligodendrocyte-type 2 astrocyte (O-2A) progenitor cells have been reported to remyelinate focal areas of demyelinated spinal cord in adult rats. We conducted a study to investigate the therapeutic potential of transplantion of O-2A cells in a rat model of acute SCI. SCI was induced with an NYU Impactor at T9 of rats. O-2A cells labeled with bromodeoxyuridine (BrdU) were transplanted into sites of SCI at 1 week after the induction of SCI. At 6 weeks after cell transplantation, a behavioral test showed significant functional improvement in animals that had received O-2A–cell transplants as compared to animals given cell-culture medium alone. An electrophysiological study revealed that the transplants did not improve the amplitude or latency of somatosensory evoked potentials, but a recording of motor evoked potentials showed that the latency of these potentials in the O-2A–cell-transplant group was significantly shorter than that in the group treated with cell-culture medium. Following transplantation of BrdU-labeled O-2A cells, cells positive for BrdU were detected at and near sites of SCI. Cells labeled for both BrdU and 2′,3′ -cyclic nucleotide-3-phosphodiesterase were also detected, showing that the transplanted O-2A cells differentiated into oligodendrocytes. By contrast, cells labeled for BrdU and glial fibrillary acidic protein, or for neuronal nuclei antigen, were not detected. Furthermore, a tract-tracing study showed that numbers of retrogradely labeled neurons increased in areas of the brain stem after O-2A–cell transplantation. The study data showed that after being transplanted into an animal with SCI, O-2A cells migrated to the area adjacent to the site of injury and differentiated into oligodendrocytes. The behavioral test and the electrophysiological and morphological studies showed that transplantation of O-2A cells may play an important role in functional recovery and the regeneration of axons after SCI.
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1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Park, Yong Gou(박용구)
Yoon, Do Heum(윤도흠) ORCID logo https://orcid.org/0000-0003-1452-5724
Lee, Bae Hwan(이배환) ORCID logo https://orcid.org/0000-0003-4719-9021
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