Cited 6 times in
Periodontal healing with a preameloblast-conditioned medium in dogs.
DC Field | Value | Language |
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dc.contributor.author | 최성호 | - |
dc.date.accessioned | 2017-02-27T08:13:00Z | - |
dc.date.available | 2017-02-27T08:13:00Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0022-3484 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/147116 | - |
dc.description.abstract | BACKGROUND AND OBJECTIVE: The predictability of conventional periodontal treatments for damaged periodontal tissue is limited, particularly on the regeneration of new cementum. As signaling molecules, a range of growth factors has been used to promote periodontal regeneration on periodontal ligament (PDL) and cementum defects. A preameloblast-conditioned medium (PA-CM) was prepared from cultured murine apical bud cells, which can differentiate into ameloblasts. We examined the effect of PA-CM on PDL cells and cementoblasts in vitro and evaluated histologically the effects of PA-CM on the regeneration of experimentally induced periodontal defects in vivo. MATERIAL AND METHODS: In vitro, the effects of PA-CM on the migration of human PDL cells were examined using a scratch wound healing assay and a transwell assay. The differentiation and mineralization potential of PA-CM-treated human PDL cells and murine cementoblastic OCCM-30 cells was examined by real-time polymerase chain reaction and Alizarin red-S staining. In vivo, six mongrel dogs (12-16 kg; 6-8 mo old) were used. Twenty-four roots were replanted with either, (i) only periodontal defects (n = 12; control group), or (ii) periodontal defects and PA-CM treatment (n = 12; experimental group). In the experimental group, the PDL and cementum between notches was removed using a Gracey curette and soaked in 0.08 mL water containing 80 μg of a PA-CM for 2 min. The dogs were killed at 4 and 8 wk post-surgery. RESULTS: The in vitro results showed that PA-CM stimulated the migration of PDL cells and promoted the differentiation and mineralization of PDL cells and cementoblasts. Real-time polymerase chain reaction analysis revealed stronger expression of Runx2, Osx, OC, Bsp and Cap mRNAs in the PA-CM-treated PDL cells and cementoblasts than those in the control cells. In vivo, newly formed PDL-like tissue and cementum-like tissue were observed partially between the root surfaces and newly formed bone in the experimental group. The regenerated PDL-like tissue in the experimental group was significantly higher than that in the control group at 8 wk (p < 0.05). The replacement resorption on the experimental group was significantly lower than that in the control group at 8 wk (p < 0.05). In addition, the amount of newly formed cementum-like tissue in the experimental group was significantly higher than that in the control group at 4 and 8 wk (p < 0.05). CONCLUSION: These results suggest that PA-CM has the potential to regenerate periodontal tissues in PDL and cementum defects. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.format.extent | 284~294 | - |
dc.language | English | - |
dc.publisher | Wiley-Blackwell | - |
dc.relation.isPartOf | JOURNAL OF PERIODONTAL RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adolescent | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Calcification, Physiologic/drug effects | - |
dc.subject.MESH | Cell Differentiation/drug effects | - |
dc.subject.MESH | Cell Migration Assays | - |
dc.subject.MESH | Cell Movement/drug effects | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Cementogenesis/drug effects | - |
dc.subject.MESH | Culture Media, Conditioned | - |
dc.subject.MESH | Dental Cementum/drug effects* | - |
dc.subject.MESH | Dental Cementum/injuries | - |
dc.subject.MESH | Dogs | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Molar, Third | - |
dc.subject.MESH | Periodontal Ligament/drug effects* | - |
dc.subject.MESH | Periodontium/drug effects | - |
dc.subject.MESH | Periodontium/injuries | - |
dc.subject.MESH | RNA, Messenger/genetics | - |
dc.subject.MESH | Real-Time Polymerase Chain Reaction | - |
dc.subject.MESH | Tooth Root/drug effects | - |
dc.subject.MESH | Tooth Root/injuries | - |
dc.subject.MESH | Tooth Root/pathology | - |
dc.subject.MESH | Wound Healing/drug effects* | - |
dc.subject.MESH | Young Adult | - |
dc.title | Periodontal healing with a preameloblast-conditioned medium in dogs. | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Dentistry | - |
dc.contributor.department | Dept. of Periodontology | - |
dc.contributor.googleauthor | S.-J. Yu | - |
dc.contributor.googleauthor | D.-S. Lee | - |
dc.contributor.googleauthor | B.-O. Kim | - |
dc.contributor.googleauthor | S.-H. Choi | - |
dc.contributor.googleauthor | J.-C. Park | - |
dc.identifier.doi | 10.1111/jre.12307 | - |
dc.contributor.localId | A04081 | - |
dc.relation.journalcode | J01696 | - |
dc.identifier.eissn | 1600-0765 | - |
dc.identifier.pmid | 26257338 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/jre.12307/abstract | - |
dc.subject.keyword | cementoblast | - |
dc.subject.keyword | periodontal healing | - |
dc.subject.keyword | regeneration | - |
dc.subject.keyword | replantation | - |
dc.contributor.alternativeName | Choi, Seong Ho | - |
dc.contributor.affiliatedAuthor | Choi, Seong Ho | - |
dc.citation.volume | 51 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 284 | - |
dc.citation.endPage | 294 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PERIODONTAL RESEARCH, Vol.51(3) : 284-294, 2016 | - |
dc.date.modified | 2017-02-24 | - |
dc.identifier.rimsid | 47147 | - |
dc.type.rims | ART | - |
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