Cited 17 times in
Characterization and Preparation of Bio-Tubular Scaffolds for Fabricating Artificial Vascular Grafts by Combining Electrospinning and a Co-Culture System
DC Field | Value | Language |
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dc.contributor.author | 박종철 | - |
dc.date.accessioned | 2017-02-24T08:14:48Z | - |
dc.date.available | 2017-02-24T08:14:48Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1598-5032 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/146596 | - |
dc.description.abstract | Tissue-engineered vascular scaffolds provide a promising solution for the replacement of diseased vascular structures. However, a major challenge lies in enhancing endothelialization, host cell ingrowth, and angiogenesis. In this study, we investigated the feasibility of developing a bio-tubular scaffold from human dermal fibroblasts (HDFs) and human umbilical vein endothelial cells (HUVEC) co-cultured on electrospun poly(L-lactide-co-ε-caprolactone) membranes to address these issues. Confluent layers of HDFs stimulated the organization of HUVECs into capillary-like networks in an indirect contact (two-dimensional) co-culture on membranes. Bio-tubular scaffolds fabricated from co-cultured membranes were either grown statically in vitro or implanted subcutaneously in severe combined immunodeficient mice for up to 4 weeks for biocompatibility evaluation and functional performance. In vitro examination of co-cultures on scaffolds showed collagen remodeling and an improvement in biomechanical properties up to day 14. Morphological analysis of in vitro grown bio-tubular scaffolds revealed good attachment and growth of both cell types. After one month, co-cultured scaffolds in vivo showed higher infiltration of host cells and collagen remodeling as compared to the HDF-seeded grafts. After 4 weeks, thin continuous layers of endothelial cells and smooth muscle cells were formed as shown by staining with an antibody specific for CD31and α-actin (α-SMA). On the contrary, HDF-seeded scaffolds remained free of α-SMA-positive cells at all time points, whereas few CD31+ cells appeared after 4 weeks. Thus, co-cultured membranes provide a solution for enhancing endothelialization, tissue regeneration, and growth in bio-tubular scaffolds and may have broader applications in regenerative medicine. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.format.extent | 131~142 | - |
dc.language | English | - |
dc.publisher | Polymer Society of Korea | - |
dc.relation.isPartOf | MACROMOLECULAR RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Characterization and Preparation of Bio-Tubular Scaffolds for Fabricating Artificial Vascular Grafts by Combining Electrospinning and a Co-Culture System | - |
dc.type | Article | - |
dc.publisher.location | Korea (South) | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Medical Engineering | - |
dc.contributor.googleauthor | Boram Lee | - |
dc.contributor.googleauthor | Muhammad Shafiq | - |
dc.contributor.googleauthor | Youngmee Jung | - |
dc.contributor.googleauthor | Jong-Chul Park | - |
dc.contributor.googleauthor | Soo Hyun Kim | - |
dc.identifier.doi | 10.1007/s13233-016-4017-5 | - |
dc.contributor.localId | A01662 | - |
dc.relation.journalcode | J02177 | - |
dc.identifier.eissn | 2092-7673 | - |
dc.identifier.url | http://link.springer.com/article/10.1007/s13233-016-4017-5 | - |
dc.subject.keyword | angiogenesis | - |
dc.subject.keyword | endothelial cells | - |
dc.subject.keyword | electrospinning | - |
dc.subject.keyword | vascular scaffolds | - |
dc.subject.keyword | PLCL | - |
dc.contributor.alternativeName | Park, Jong Chul | - |
dc.contributor.affiliatedAuthor | Park, Jong Chul | - |
dc.citation.volume | 24 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 131 | - |
dc.citation.endPage | 142 | - |
dc.identifier.bibliographicCitation | MACROMOLECULAR RESEARCH, Vol.24(2) : 131-142, 2016 | - |
dc.date.modified | 2017-02-24 | - |
dc.identifier.rimsid | 46405 | - |
dc.type.rims | ART | - |
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