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Characterization and Preparation of Bio-Tubular Scaffolds for Fabricating Artificial Vascular Grafts by Combining Electrospinning and a Co-Culture System

DC FieldValueLanguage
dc.contributor.author박종철-
dc.date.accessioned2017-02-24T08:14:48Z-
dc.date.available2017-02-24T08:14:48Z-
dc.date.issued2016-
dc.identifier.issn1598-5032-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/146596-
dc.description.abstractTissue-engineered vascular scaffolds provide a promising solution for the replacement of diseased vascular structures. However, a major challenge lies in enhancing endothelialization, host cell ingrowth, and angiogenesis. In this study, we investigated the feasibility of developing a bio-tubular scaffold from human dermal fibroblasts (HDFs) and human umbilical vein endothelial cells (HUVEC) co-cultured on electrospun poly(L-lactide-co-ε-caprolactone) membranes to address these issues. Confluent layers of HDFs stimulated the organization of HUVECs into capillary-like networks in an indirect contact (two-dimensional) co-culture on membranes. Bio-tubular scaffolds fabricated from co-cultured membranes were either grown statically in vitro or implanted subcutaneously in severe combined immunodeficient mice for up to 4 weeks for biocompatibility evaluation and functional performance. In vitro examination of co-cultures on scaffolds showed collagen remodeling and an improvement in biomechanical properties up to day 14. Morphological analysis of in vitro grown bio-tubular scaffolds revealed good attachment and growth of both cell types. After one month, co-cultured scaffolds in vivo showed higher infiltration of host cells and collagen remodeling as compared to the HDF-seeded grafts. After 4 weeks, thin continuous layers of endothelial cells and smooth muscle cells were formed as shown by staining with an antibody specific for CD31and α-actin (α-SMA). On the contrary, HDF-seeded scaffolds remained free of α-SMA-positive cells at all time points, whereas few CD31+ cells appeared after 4 weeks. Thus, co-cultured membranes provide a solution for enhancing endothelialization, tissue regeneration, and growth in bio-tubular scaffolds and may have broader applications in regenerative medicine.-
dc.description.statementOfResponsibilityrestriction-
dc.format.extent131~142-
dc.languageEnglish-
dc.publisherPolymer Society of Korea-
dc.relation.isPartOfMacromolecular Research-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleCharacterization and Preparation of Bio-Tubular Scaffolds for Fabricating Artificial Vascular Grafts by Combining Electrospinning and a Co-Culture System-
dc.typeArticle-
dc.publisher.locationKorea (South)-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Medical Engineering-
dc.contributor.googleauthorBoram Lee-
dc.contributor.googleauthorMuhammad Shafiq-
dc.contributor.googleauthorYoungmee Jung-
dc.contributor.googleauthorJong-Chul Park-
dc.contributor.googleauthorSoo Hyun Kim-
dc.identifier.doi10.1007/s13233-016-4017-5-
dc.contributor.localIdA01662-
dc.relation.journalcodeJ02177-
dc.identifier.urlhttp://link.springer.com/article/10.1007/s13233-016-4017-5-
dc.subject.keywordangiogenesis-
dc.subject.keywordendothelial cells-
dc.subject.keywordelectrospinning-
dc.subject.keywordvascular scaffolds-
dc.subject.keywordPLCL-
dc.contributor.alternativeNamePark, Jong Chul-
dc.contributor.affiliatedAuthorPark, Jong Chul-
dc.citation.volume24-
dc.citation.number2-
dc.citation.startPage131-
dc.citation.endPage142-
dc.identifier.bibliographicCitationMacromolecular Research, Vol.24(2) : 131-142, 2016-
dc.date.modified2017-02-24-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers

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