665 954

Cited 8 times in

Standardization of Weed Pollen Extracts, Japanese Hop and Mugwort, in Korea

DC Field Value Language
dc.contributor.author박경희-
dc.contributor.author박중원-
dc.contributor.author박혜정-
dc.contributor.author이재현-
dc.contributor.author정경용-
dc.contributor.author홍천수-
dc.date.accessioned2017-02-24T07:34:31Z-
dc.date.available2017-02-24T07:34:31Z-
dc.date.issued2016-
dc.identifier.issn0513-5796-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/146459-
dc.description.abstractPURPOSE: Japanese hop (Humulus spp.) and mugwort (Artemisia spp.) are notable causes of autumn pollinosis in East Asia. However, Japanese hop and mugwort pollen extracts, which are widely used for the diagnosis, have not been standardized. This study was performed to standardize Japanese hop and mugwort pollen extracts. MATERIALS AND METHODS: Allergen extracts were prepared in a standardized way using locally collected Humulus japonicus and purchased Artemisia vulgaris pollens. The immunoglobulin E (IgE) reactivities of prepared extracts were compared with commercial extracts via IgE immunoblotting and inhibition analyses. Intradermal skin tests were performed to determine the bioequivalent allergy unit (BAU). RESULTS: The IgE reactive components of the extracts via IgE immunoblotting were similar to those of commercial extracts. A 11-kDa allergen showed the strongest IgE reactivity in Japanese hop, as did a 28-kDa allergen in mugwort pollen extracts. Allergenic potencies of the investigatory Japanese hop and mugwort extracts were essentially indistinguishable from the commercial ones. Sums of erythema of 50 mm by the intradermal skin test (ΣED50) were calculated to be 14.4th and 13.6th three-fold dilutions for Japanese hop and mugwort extracts, respectively. Therefore, the allergenic activity of the prepared extracts was 90827.4 BAU/mg for Japanese hop and 34412 BAU/mg for mugwort. CONCLUSION: We produced Japanese hop and mugwort pollen extracts using a standardized method. Standardized Japanese hop and mugwort pollen extracts will facilitate the production of improved diagnostic and immunotherapeutic reagents.-
dc.description.statementOfResponsibilityopen-
dc.format.extent399~406-
dc.languageEnglish-
dc.publisherYonsei University-
dc.relation.isPartOfYONSEI MEDICAL JOURNAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAllergens/analysis*-
dc.subject.MESHAllergens/immunology*-
dc.subject.MESHAntibody Specificity-
dc.subject.MESHArtemisia*-
dc.subject.MESHBronchial Hyperreactivity/blood-
dc.subject.MESHBronchial Hyperreactivity/immunology-
dc.subject.MESHCross Reactions-
dc.subject.MESHEnzyme-Linked Immunosorbent Assay-
dc.subject.MESHHumans-
dc.subject.MESHImmunoblotting-
dc.subject.MESHImmunoglobulin E/blood-
dc.subject.MESHImmunoglobulin E/immunology*-
dc.subject.MESHPollen/chemistry*-
dc.subject.MESHPollen/immunology*-
dc.subject.MESHReference Standards-
dc.subject.MESHRepublic of Korea-
dc.subject.MESHRhinitis, Allergic, Seasonal-
dc.titleStandardization of Weed Pollen Extracts, Japanese Hop and Mugwort, in Korea-
dc.typeArticle-
dc.publisher.locationKorea (South)-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Internal Medicine-
dc.contributor.googleauthorKyoung Yong Jeong-
dc.contributor.googleauthorMina Son-
dc.contributor.googleauthorSoo-Young Choi-
dc.contributor.googleauthorKyung Hee Park-
dc.contributor.googleauthorHye Jung Park-
dc.contributor.googleauthorChein-Soo Hong-
dc.contributor.googleauthorJae-Hyun Lee-
dc.contributor.googleauthorJung-Won Park-
dc.identifier.doi10.3349/ymj.2016.57.2.399-
dc.contributor.localIdA01427-
dc.contributor.localIdA01681-
dc.contributor.localIdA01769-
dc.contributor.localIdA03086-
dc.contributor.localIdA03572-
dc.contributor.localIdA04448-1-
dc.relation.journalcodeJ02813-
dc.identifier.eissn1976-2437-
dc.identifier.pmid26847293-
dc.subject.keywordAllergen standardization-
dc.subject.keywordJapanese hop-
dc.subject.keywordmugwort-
dc.contributor.alternativeNamePark, Kyung Hee-
dc.contributor.alternativeNamePark, Jung Won-
dc.contributor.alternativeNamePark, Hye Jung-
dc.contributor.alternativeNameLee, Jae Hyun-
dc.contributor.alternativeNameJeong, Kyoung Yong-
dc.contributor.alternativeNameHong, Chein Soo-
dc.contributor.affiliatedAuthorPark, Kyung Hee-
dc.contributor.affiliatedAuthorPark, Jung Won-
dc.contributor.affiliatedAuthorPark, Hye Jung-
dc.contributor.affiliatedAuthorLee, Jae Hyun-
dc.contributor.affiliatedAuthorJeong, Kyoung Yong-
dc.contributor.affiliatedAuthorHong, Chein Soo-
dc.citation.volume57-
dc.citation.number2-
dc.citation.startPage399-
dc.citation.endPage406-
dc.identifier.bibliographicCitationYONSEI MEDICAL JOURNAL, Vol.57(2) : 399-406, 2016-
dc.date.modified2017-02-24-
dc.identifier.rimsid45103-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.