Cited 9 times in
Standardization of Weed Pollen Extracts, Japanese Hop and Mugwort, in Korea
DC Field | Value | Language |
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dc.contributor.author | 박경희 | - |
dc.contributor.author | 박중원 | - |
dc.contributor.author | 박혜정 | - |
dc.contributor.author | 이재현 | - |
dc.contributor.author | 정경용 | - |
dc.contributor.author | 홍천수 | - |
dc.date.accessioned | 2017-02-24T07:34:31Z | - |
dc.date.available | 2017-02-24T07:34:31Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/146459 | - |
dc.description.abstract | PURPOSE: Japanese hop (Humulus spp.) and mugwort (Artemisia spp.) are notable causes of autumn pollinosis in East Asia. However, Japanese hop and mugwort pollen extracts, which are widely used for the diagnosis, have not been standardized. This study was performed to standardize Japanese hop and mugwort pollen extracts. MATERIALS AND METHODS: Allergen extracts were prepared in a standardized way using locally collected Humulus japonicus and purchased Artemisia vulgaris pollens. The immunoglobulin E (IgE) reactivities of prepared extracts were compared with commercial extracts via IgE immunoblotting and inhibition analyses. Intradermal skin tests were performed to determine the bioequivalent allergy unit (BAU). RESULTS: The IgE reactive components of the extracts via IgE immunoblotting were similar to those of commercial extracts. A 11-kDa allergen showed the strongest IgE reactivity in Japanese hop, as did a 28-kDa allergen in mugwort pollen extracts. Allergenic potencies of the investigatory Japanese hop and mugwort extracts were essentially indistinguishable from the commercial ones. Sums of erythema of 50 mm by the intradermal skin test (ΣED50) were calculated to be 14.4th and 13.6th three-fold dilutions for Japanese hop and mugwort extracts, respectively. Therefore, the allergenic activity of the prepared extracts was 90827.4 BAU/mg for Japanese hop and 34412 BAU/mg for mugwort. CONCLUSION: We produced Japanese hop and mugwort pollen extracts using a standardized method. Standardized Japanese hop and mugwort pollen extracts will facilitate the production of improved diagnostic and immunotherapeutic reagents. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 399~406 | - |
dc.language | English | - |
dc.publisher | Yonsei University | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Allergens/analysis* | - |
dc.subject.MESH | Allergens/immunology* | - |
dc.subject.MESH | Antibody Specificity | - |
dc.subject.MESH | Artemisia* | - |
dc.subject.MESH | Bronchial Hyperreactivity/blood | - |
dc.subject.MESH | Bronchial Hyperreactivity/immunology | - |
dc.subject.MESH | Cross Reactions | - |
dc.subject.MESH | Enzyme-Linked Immunosorbent Assay | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunoblotting | - |
dc.subject.MESH | Immunoglobulin E/blood | - |
dc.subject.MESH | Immunoglobulin E/immunology* | - |
dc.subject.MESH | Pollen/chemistry* | - |
dc.subject.MESH | Pollen/immunology* | - |
dc.subject.MESH | Reference Standards | - |
dc.subject.MESH | Republic of Korea | - |
dc.subject.MESH | Rhinitis, Allergic, Seasonal | - |
dc.title | Standardization of Weed Pollen Extracts, Japanese Hop and Mugwort, in Korea | - |
dc.type | Article | - |
dc.publisher.location | Korea (South) | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Internal Medicine | - |
dc.contributor.googleauthor | Kyoung Yong Jeong | - |
dc.contributor.googleauthor | Mina Son | - |
dc.contributor.googleauthor | Soo-Young Choi | - |
dc.contributor.googleauthor | Kyung Hee Park | - |
dc.contributor.googleauthor | Hye Jung Park | - |
dc.contributor.googleauthor | Chein-Soo Hong | - |
dc.contributor.googleauthor | Jae-Hyun Lee | - |
dc.contributor.googleauthor | Jung-Won Park | - |
dc.identifier.doi | 10.3349/ymj.2016.57.2.399 | - |
dc.contributor.localId | A01427 | - |
dc.contributor.localId | A01681 | - |
dc.contributor.localId | A01769 | - |
dc.contributor.localId | A03086 | - |
dc.contributor.localId | A03572 | - |
dc.contributor.localId | A04448-1 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 26847293 | - |
dc.subject.keyword | Allergen standardization | - |
dc.subject.keyword | Japanese hop | - |
dc.subject.keyword | mugwort | - |
dc.contributor.alternativeName | Park, Kyung Hee | - |
dc.contributor.alternativeName | Park, Jung Won | - |
dc.contributor.alternativeName | Park, Hye Jung | - |
dc.contributor.alternativeName | Lee, Jae Hyun | - |
dc.contributor.alternativeName | Jeong, Kyoung Yong | - |
dc.contributor.alternativeName | Hong, Chein Soo | - |
dc.contributor.affiliatedAuthor | Park, Kyung Hee | - |
dc.contributor.affiliatedAuthor | Park, Jung Won | - |
dc.contributor.affiliatedAuthor | Park, Hye Jung | - |
dc.contributor.affiliatedAuthor | Lee, Jae Hyun | - |
dc.contributor.affiliatedAuthor | Jeong, Kyoung Yong | - |
dc.contributor.affiliatedAuthor | Hong, Chein Soo | - |
dc.citation.volume | 57 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 399 | - |
dc.citation.endPage | 406 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.57(2) : 399-406, 2016 | - |
dc.date.modified | 2017-02-24 | - |
dc.identifier.rimsid | 45103 | - |
dc.type.rims | ART | - |
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