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Knockdown of HIF-1a and IL-8 induced apoptosis of hepatocellular carcinoma triggers apoptosis of vascular endothelial cells

DC FieldValueLanguage
dc.contributor.author강원석-
dc.contributor.author박준용-
dc.contributor.author안상훈-
dc.contributor.author한광협-
dc.contributor.author김도영-
dc.contributor.author김승업-
dc.contributor.author노원상-
dc.date.accessioned2017-02-24T03:15:31Z-
dc.date.available2017-02-24T03:15:31Z-
dc.date.issued2016-
dc.identifier.issn1360-8185-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/146291-
dc.description.abstractA local hypoxic microenvironment is one of the most important characteristics of solid tumors. Hypoxia inducible factor-1α (HIF-1α) and Interleukin-8 (IL-8) activate tumor survival from hypoxic-induced apoptosis in each pathway. This study aimed to evaluate whether knockdown of HIF-1α and IL-8 induced apoptosis of the hepatocellular carcinoma (HCC) and endothelial cell lines. HCC cell lines were infected with adenovirus-expressing shRNA for HIF-1α and IL-8 and maintained under hypoxic conditions (1% O2, 24 h). The expression levels of HIF-1α and both apoptotic and growth factors were examined by real-time quantitative PCR and western blot. We also investigated apoptosis by TUNEL assay (FACS and Immunofluorescence) and measured the concentration of cytochrome C. Inhibition of HIF-1α and IL-8 up-regulated the expression of apoptotic factors while downregulating anti-apoptotic factors simultaneously. Knockdown of HIF-1α and IL-8 increased the concentration of cytochrome C and enhanced DNA fragmentation in HCC cell lines. Moreover, culture supernatant collected from the knockdown of HIF-1α and IL-8 in HCC cell lines induced apoptosis in human umbilical vein endothelial cells under hypoxia, and the expression of variable apoptotic ligand increased from HCC cell lines, time-dependently. These data suggest that adenovirus-mediated knockdown of HIF-1α and IL-8 induced apoptosis in HCC cells and triggered apoptosis of vascular endothelial cells.-
dc.description.statementOfResponsibilityrestriction-
dc.format.extent85~95-
dc.publisherSpringer-
dc.relation.isPartOfApoptosis-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdenoviridae/genetics-
dc.subject.MESHAdenoviridae/metabolism-
dc.subject.MESHApoptosis/drug effects*-
dc.subject.MESHApoptosis/genetics-
dc.subject.MESHCaspase 3/genetics-
dc.subject.MESHCaspase 3/metabolism-
dc.subject.MESHCell Hypoxia-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCulture Media, Conditioned/pharmacology-
dc.subject.MESHCytochromes c/secretion-
dc.subject.MESHDNA Fragmentation-
dc.subject.MESHGene Expression Regulation, Neoplastic*-
dc.subject.MESHGenetic Vectors/chemistry-
dc.subject.MESHGenetic Vectors/metabolism-
dc.subject.MESHHep G2 Cells-
dc.subject.MESHHuman Umbilical Vein Endothelial Cells/cytology-
dc.subject.MESHHuman Umbilical Vein Endothelial Cells/drug effects-
dc.subject.MESHHuman Umbilical Vein Endothelial Cells/metabolism-
dc.subject.MESHHumans-
dc.subject.MESHHypoxia-Inducible Factor 1, alpha Subunit/antagonists & inhibitors-
dc.subject.MESHHypoxia-Inducible Factor 1, alpha Subunit/genetics*-
dc.subject.MESHHypoxia-Inducible Factor 1, alpha Subunit/metabolism-
dc.subject.MESHInterleukin-8/antagonists & inhibitors-
dc.subject.MESHInterleukin-8/genetics*-
dc.subject.MESHInterleukin-8/metabolism-
dc.subject.MESHRNA, Small Interfering/genetics-
dc.subject.MESHRNA, Small Interfering/metabolism-
dc.subject.MESHSignal Transduction-
dc.subject.MESHbcl-X Protein/genetics*-
dc.subject.MESHbcl-X Protein/metabolism-
dc.titleKnockdown of HIF-1a and IL-8 induced apoptosis of hepatocellular carcinoma triggers apoptosis of vascular endothelial cells-
dc.typeArticle-
dc.publisher.locationNetherlands-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Internal Medicine-
dc.contributor.googleauthorSung Hoon Choi-
dc.contributor.googleauthorJun Yong Park-
dc.contributor.googleauthorWonseok Kang-
dc.contributor.googleauthorSeung Up Kim-
dc.contributor.googleauthorDo Young Kim-
dc.contributor.googleauthorSang Hoon Ahn-
dc.contributor.googleauthorSimon Wonsang Ro-
dc.contributor.googleauthorKwang-Hyub Han-
dc.identifier.doi10.1007/s10495-015-1185-2-
dc.contributor.localIdA00061-
dc.contributor.localIdA01675-
dc.contributor.localIdA02226-
dc.contributor.localIdA04268-
dc.contributor.localIdA00385-
dc.contributor.localIdA00654-
dc.contributor.localIdA01286-
dc.relation.journalcodeJ00195-
dc.identifier.pmid26467924-
dc.identifier.urlhttp://link.springer.com/article/10.1007/s10495-015-1185-2-
dc.subject.keywordApoptosis-
dc.subject.keywordDeath signal ligands-
dc.subject.keywordFACs-TUNEL-
dc.subject.keywordHypoxia inducible factor-1α-
dc.subject.keywordInterleukin-8-
dc.subject.keywordTumor xenograft model-
dc.contributor.alternativeNameKang, Won Suk-
dc.contributor.alternativeNamePark, Jun Yong-
dc.contributor.alternativeNameAhn, Sang Hoon-
dc.contributor.alternativeNameHan, Kwang Hyup-
dc.contributor.alternativeNameKim, Do Young-
dc.contributor.alternativeNameKim, Seung Up-
dc.contributor.alternativeNameRo, Simon Weonsang-
dc.contributor.affiliatedAuthorKang, Won Suk-
dc.contributor.affiliatedAuthorPark, Jun Yong-
dc.contributor.affiliatedAuthorAhn, Sang Hoon-
dc.contributor.affiliatedAuthorHan, Kwang Hyup-
dc.contributor.affiliatedAuthorKim, Do Young-
dc.contributor.affiliatedAuthorKim, Seung Up-
dc.contributor.affiliatedAuthorRo, Simon Weonsang-
dc.citation.volume21-
dc.citation.number1-
dc.citation.startPage85-
dc.citation.endPage95-
dc.identifier.bibliographicCitationApoptosis, Vol.21(1) : 85-95, 2016-
dc.date.modified2017-02-24-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Liver Cirrhosis Clinical Research Center (간경변증임상연구센터) > 1. Journal Papers

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