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Rapid detection of Pseudomonas aeruginosa and Acinetobacter baumannii Harboring bla(VIM-2), bla(IMP-1) and bla(OXA-23) genes by using loop-mediated isothermal amplification methods.

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dc.contributor.author용동은-
dc.contributor.author윤상선-
dc.contributor.author이재면-
dc.date.accessioned2017-02-24T03:09:22Z-
dc.date.available2017-02-24T03:09:22Z-
dc.date.issued2016-
dc.identifier.issn2234-3806-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/146262-
dc.description.abstractBACKGROUND: Carbapenem-resistant Pseudomonas aeruginosa (CRPA) and Acinetobacter baumannii (CRAB) are the leading causes of nosocomial infections. A rapid and sensitive test to detect CRPA and CRAB is required for appropriate antibiotic treatment. We optimized a loop-mediated isothermal amplification (LAMP) assay to detect the presence of bla(VIM-2), bla(IMP-1), and bla(OXA-23), which are critical components for carbapenem resistance. METHODS: Two sets of primers, inner and outer primers, were manually designed as previously described. The LAMP buffer was optimized (at 2mM MgSO₄) by testing different concentrations of MgSO₄. The optimal reaction temperature and incubation time were determined by using a gradient thermocycler. Then, the optimized bla(VIM-2), bla(IMP-1), and bla(OXA-23) LAMP reactions were evaluated by using 120 P. aeruginosa and 99 A. baumannii clinical isolates. RESULTS: Only one strain of the 100 CRPA isolates harbored bla(IMP-1), whereas none of them harbored bla(VIM-2). These results indicate that the acquisition of bla(VIM-2) or bla(IMP-1) may not play a major role in carbapenem resistance in Korea. Fifty two strains of the 75 CRAB isolates contained bla(OXA-23), but none contained bla(VIM-2) and bla(IMP-1) alleles. CONCLUSIONS: Our results demonstrate the usefulness of LAMP for the diagnosis of CRPA and CRAB.-
dc.description.statementOfResponsibilityopen-
dc.format.extent15~22-
dc.languageEnglish-
dc.publisherKorean Society for Laboratory Medicine-
dc.relation.isPartOfANNALS OF LABORATORY MEDICINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAcinetobacter baumannii/genetics-
dc.subject.MESHAcinetobacter baumannii/isolation & purification*-
dc.subject.MESHAnti-Bacterial Agents/pharmacology*-
dc.subject.MESHCarbapenems/pharmacology*-
dc.subject.MESHDrug Resistance, Bacterial/genetics*-
dc.subject.MESHGenes, Bacterial*-
dc.subject.MESHNucleic Acid Amplification Techniques-
dc.subject.MESHPseudomonas aeruginosa/genetics-
dc.subject.MESHPseudomonas aeruginosa/isolation & purification*-
dc.subject.MESHSensitivity and Specificity-
dc.titleRapid detection of Pseudomonas aeruginosa and Acinetobacter baumannii Harboring bla(VIM-2), bla(IMP-1) and bla(OXA-23) genes by using loop-mediated isothermal amplification methods.-
dc.typeArticle-
dc.publisher.locationKorea-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Laboratory Medicine-
dc.contributor.googleauthorHye Jin Kim-
dc.contributor.googleauthorHyung Sun Kim-
dc.contributor.googleauthorJae Myun Lee-
dc.contributor.googleauthorSang Sun Yoon-
dc.contributor.googleauthorDongeun Yong-
dc.identifier.doi10.3343/alm.2016.36.1.15-
dc.contributor.localIdA02423-
dc.contributor.localIdA02558-
dc.contributor.localIdA03071-
dc.relation.journalcodeJ00164-
dc.identifier.eissn2234-3814-
dc.relation.journalsince2012~-
dc.identifier.pmid26522754-
dc.relation.journalbefore~2011 Korean Journal of Laboratory Medicine (대한진단검사의학회지)-
dc.subject.keywordAcinetobacter baumannii-
dc.subject.keywordCarbapenem resistance-
dc.subject.keywordLoop-mediated isothermal amplification (LAMP)-
dc.subject.keywordPseudomonas aeruginosa-
dc.subject.keywordbla(IMP-1)-
dc.subject.keywordbla(OXA-23)-
dc.subject.keywordbla(VIM-2)-
dc.contributor.alternativeNameYong, Dong Eun-
dc.contributor.alternativeNameYoon, Sang Sun-
dc.contributor.alternativeNameLee, Jae Myun-
dc.contributor.affiliatedAuthorYong, Dong Eun-
dc.contributor.affiliatedAuthorYoon, Sang Sun-
dc.contributor.affiliatedAuthorLee, Jae Myun-
dc.citation.volume36-
dc.citation.number1-
dc.citation.startPage15-
dc.citation.endPage22-
dc.identifier.bibliographicCitationANNALS OF LABORATORY MEDICINE, Vol.36(1) : 15-22, 2016-
dc.date.modified2017-02-24-
dc.identifier.rimsid51299-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers

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