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추간판내 유전자 전달에 의한 전신 면역 반응

Other Titles
 Host Immune Responses to Intradiscal Gene Transfer 
Authors
 문성환  ;  김향  ;  권언혜  ;  박의현  ;  이환모  ;  한수봉  ;  김남현 
Citation
 Journal of Korean Society of Spine Surgery (대한척추외과학회지), Vol.9(2) : 84-90, 2002 
Journal Title
 Journal of Korean Society of Spine Surgery (대한척추외과학회지) 
ISSN
 1229-5701 
Issue Date
2002
Keywords
Intradiscal Gene Therapy ; Immune Responses
Abstract
Purpose To elucidate host immune responses to intradiscal gene transfer. Materials and Methods Twenty rabbits were utilized. Ad/luciferase (adenovirus construct) were injected into nucleus pulposus of lumbar vertebrae. Group 1 received intradiscal injection of Ad/luciferase only, Group 2 received subcutaneous and intradiscal injection simultaneously, Group 3 received subcutaneous injection then intradiscal injection with 2 weeks interval. Blood samples were obtained serially after injection. Animals were sacrificed at 7 weeks. Antibody to adenovirus in peripheral blood was measured with ELISA and transgene expression was measured with standard luciferase kits. Results All rabbits in the Group 2 and 3 exhibited increased production of neutralizing antibody. There were clearly two subgroups in Group 1, three rabbits exhibited production of antibody but remaining three rabbits showed little or no production of antibody. All rabbits showed robust increase in transgene expression regardless of titer of neutralizing antibody. Conclusion The intervertebral disc is favorable site for adenovirus-mediated gene transfer escaping from systemic immunity.
Files in This Item:
T200211015.pdf Download
DOI
10.4184/jkss.2002.9.2.84
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Nam Hyun(김남현)
Moon, Seong Hwan(문성환)
Lee, Hwan Mo(이환모) ORCID logo https://orcid.org/0000-0002-5405-3832
Hahn, Soo Bong(한수봉)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/144724
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