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Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linking

DC Field Value Language
dc.contributor.author박종철-
dc.contributor.author서활-
dc.date.accessioned2016-05-16T11:28:09Z-
dc.date.available2016-05-16T11:28:09Z-
dc.date.issued2002-
dc.identifier.issn0142-9612-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/144618-
dc.description.abstractIn order to develop a scaffolding material for tissue regeneration, porous matrices containing collagen and hyaluronic acid were fabricated by freeze drying at -20 degrees C, -70 degrees C or -196 degrees C. The fabricated porous membranes were cross-linked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) in a range of 1-100 mM concentrations for enhancing mechanical stability of the composite matrix. Scanning electron microscope (SEM) views of the matrices demonstrated that the matrices obtained before cross-linking process had interconnected pores with mean diameters of 40, 90 or 230 microm and porosity of 58-66% according to the freezing temperature, and also the porous structures after cross-linking process were retained. The swelling test and IR spectroscopic measurement of different cross-linked membranes were carried out as a measure of the extent of cross-linking. The swelling behavior of cross-linked membranes showed no significant differences as cross-linking degree increased. FT-IR spectra showed the increase of the intensity of the absorbencies at amide bonds (1655, 1546, 1458 cm(-1)) compared to that of CH bond (2930 cm(-1)). In enzymatic degradation test, EDC treated membranes showed significant enhancement of the resistance to collagenase activity in comparison with 0.625% glutaraldehyde treated membranes. In cytotoxicity test using L929 fibroblastic cells, the EDC-cross-linked membranes demonstrated no significant toxicity.-
dc.description.statementOfResponsibilityopen-
dc.format.extent1205~1212-
dc.relation.isPartOfBIOMATERIALS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleCharacterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linking-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Medical Engineering (의학공학)-
dc.contributor.googleauthorSi-Nae Park-
dc.contributor.googleauthorJong-Chul Park-
dc.contributor.googleauthorHea Ok Kim-
dc.contributor.googleauthorMin Jung Song-
dc.contributor.googleauthorHwal Suh-
dc.identifier.doi10.1016/S0142-9612(01)00235-6-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01662-
dc.contributor.localIdA01924-
dc.relation.journalcodeJ00312-
dc.identifier.eissn1878-5905-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0142961201002356-
dc.subject.keywordCollagen-
dc.subject.keywordHyaluronic acid-
dc.subject.keywordPorous scaffold-
dc.subject.keywordEDC cross-linking-
dc.contributor.alternativeNamePark, Jong Chul-
dc.contributor.alternativeNameSuh, Hwal-
dc.contributor.affiliatedAuthorPark, Jong Chul-
dc.contributor.affiliatedAuthorSuh, Hwal-
dc.rights.accessRightsnot free-
dc.citation.volume23-
dc.citation.number4-
dc.citation.startPage1205-
dc.citation.endPage1212-
dc.identifier.bibliographicCitationBIOMATERIALS, Vol.23(4) : 1205-1212, 2002-
dc.identifier.rimsid51303-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers

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