α-MSH와 α-MSH 효현제가 사람 말초혈액 단구에서 유래한 수지상세포의 표면항원 표현에 미치는 영향

Abstract

There is an accumulating evidence that α-MSH, besides being a pigmentary hormone also plays a crucial role in the regulation of immune and inflammatory reactions. Alpha-MSH might exert its immunomodulatory effects by downregulating accessory molecules on antigen-presenting cells. Dendritic cells (DC) are a system of highly efficient antigen-presenting cells that initiate the primary immune response. Recently, new two-step culture system for immature/mature DC generation from human peripheral blood-derived monocytes was established. In this study, we generated monocyte-derived immature/mature DC to the two-step culture system and observed the changes of surface molecule of human monocyte-derived dendritic cells with α-MSH and its agonist by flow cytometry. The following results were obtained: 1. α-MSH suppressed the expression of B7-2 and HLA-DR in immature DCs. 2. α-MSH suppressed the expression of HLA-DR in mature DCs. 3. α-MSH-ND (α-MSH agonist) also suppressed the expression of B7-2 and HLA-DR in mature DCs. From the result, it is suggested that α-MSH and its agonist α-MSH-ND are capable of downregulating immune response through suppressing accessory molecules on antigen presenting cells.