Cited 25 times in
Mechanism of silica-induced ROS generation in Rat2 fibroblast cells
DC Field | Value | Language |
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dc.contributor.author | 김형중 | - |
dc.contributor.author | 이운규 | - |
dc.date.accessioned | 2016-05-16T11:25:07Z | - |
dc.date.available | 2016-05-16T11:25:07Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0378-4274 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/144506 | - |
dc.description.abstract | Reactive oxygen species (ROS) play an important role in cell signaling pathway. Previously, we found that silica induced immediate ROS generation and sequential cellular responses such as kinase activation in Rat2 cells as well as an increase of intracellular calcium concentration in A549 cells. However, the detailed mechanism underlying the immediate ROS generation induced by silica in fibroblast cells remains to be elucidated. Therefore, in the present study, we investigated the mechanism of ROS generation by silica within Rat2 fibroblast cells by examining the effects of a diverse group of inhibitors for the enzymes related with signal transduction events. Inhibitors for protein tyrosine kinase (PTK), phospholipase C (PLC), protein kinase C (PKC) and calmodulin (CaM) kinase II effectively suppressed ROS generation in silica-stimulated Rat2 cells, whereas those for protein kinase A and phospholipase A2 did not. Diphenyleneiodonium chloride (DPI), an inhibitor for NADPH oxidase was also found to be effective in inhibiting silica-induced ROS generation. These results suggest that PTK, PLC, PKC, CaM kinase II, and NADPH oxidase are all involved in signal transduction pathways for ROS generation in silica-stimulated Rat2 cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 185~191 | - |
dc.relation.isPartOf | TOXICOLOGY LETTERS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Dose-Response Relationship, Drug | - |
dc.subject.MESH | Fibroblasts/drug effects* | - |
dc.subject.MESH | Fibroblasts/metabolism* | - |
dc.subject.MESH | Genistein/pharmacology | - |
dc.subject.MESH | Microscopy, Fluorescence | - |
dc.subject.MESH | Protein Kinase Inhibitors | - |
dc.subject.MESH | Protein Kinases/metabolism | - |
dc.subject.MESH | Quinacrine/pharmacology | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Reactive Oxygen Species/metabolism* | - |
dc.subject.MESH | Signal Transduction/drug effects | - |
dc.subject.MESH | Silicon Dioxide/pharmacology* | - |
dc.subject.MESH | Silicon Dioxide/toxicity | - |
dc.subject.MESH | Type C Phospholipases/antagonists & inhibitors | - |
dc.subject.MESH | Type C Phospholipases/metabolism | - |
dc.title | Mechanism of silica-induced ROS generation in Rat2 fibroblast cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Medical Research Center (임상의학연구센터) | - |
dc.contributor.googleauthor | Kyoung-Ah Kim | - |
dc.contributor.googleauthor | Young-Hoon Kim | - |
dc.contributor.googleauthor | Min Seok Seo | - |
dc.contributor.googleauthor | Woon Kyu Lee | - |
dc.contributor.googleauthor | Seung Won Kim | - |
dc.contributor.googleauthor | Hongtae Kim | - |
dc.contributor.googleauthor | Kweon-Haeng Lee | - |
dc.contributor.googleauthor | In-Chul Shin | - |
dc.contributor.googleauthor | Joong-Soo Han | - |
dc.contributor.googleauthor | Hyoung Joong Kimf | - |
dc.contributor.googleauthor | Young Lim | - |
dc.identifier.doi | 10.1016/S0378-4274(02)00237-0 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01158 | - |
dc.contributor.localId | A02996 | - |
dc.relation.journalcode | J02744 | - |
dc.identifier.eissn | 1879-3169 | - |
dc.identifier.pmid | 12270676 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0378427402002370 | - |
dc.subject.keyword | Silica | - |
dc.subject.keyword | Fibroblast | - |
dc.subject.keyword | ROS | - |
dc.subject.keyword | PTK | - |
dc.subject.keyword | PLC | - |
dc.subject.keyword | PKC | - |
dc.subject.keyword | CaM kinase II | - |
dc.contributor.alternativeName | Kim, Hyung Jung | - |
dc.contributor.alternativeName | Lee, Woon Kyu | - |
dc.contributor.affiliatedAuthor | Kim, Hyung Jung | - |
dc.contributor.affiliatedAuthor | Lee, Woon Kyu | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 135 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 185 | - |
dc.citation.endPage | 191 | - |
dc.identifier.bibliographicCitation | TOXICOLOGY LETTERS, Vol.135(3) : 185-191, 2002 | - |
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