Cited 43 times in
Molecular mechanism of the activation-induced cell death inhibition mediated p70 inhibitory killer cell Ig-like receptor in Jurkat T cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김세종 | - |
dc.contributor.author | 김종선 | - |
dc.date.accessioned | 2016-05-16T11:24:45Z | - |
dc.date.available | 2016-05-16T11:24:45Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/144492 | - |
dc.description.abstract | In this study we investigated the molecular mechanism of the activation-induced cell death (AICD) inhibition mediated by a p70 inhibitory killer cell Ig-like receptor (KIR3DL1, also called NKB1) in Jurkat T cells. Using stable Jurkat transfectants that express KIR or CD8-KIR fusion proteins we have shown for the first time that KIR inhibits, in a ligation-independent manner, the AICD induced by PHA, PMA/ionomycin, or anti-CD3 Ab. The AICD inhibition mediated by KIR appears to result from the blockade of Fas ligand induction upon activation of the Jurkat transfectants. Moreover, the membrane-proximal 20 aa of the KIR cytoplasmic tail were determined to play a crucial role in this process. Since the membrane-proximal portion of the KIR cytoplasmic tail contains a putative protein kinase C (PKC) substrate site, we investigated the molecular interaction between KIR and PKC. Immunoprecipitation analysis demonstrated that KIR constitutively bound both to PKCα, a conventional Ca2+-dependent PKC, and to PKCθ, a novel Ca2+-independent PKC. Furthermore, an in vitro kinase assay revealed that PKC activation was blocked after PHA stimulation in Jurkat transfectants expressing KIR. These observations were supported by the finding that a recombinant KIR cytoplasmic tail also appeared to inhibit PKCα activation in vitro. Taken together these data strongly suggest that KIR inhibits the AICD of T cells by blocking Fas ligand induction upon stimulation, in a process that seems to be accomplished by PKC recruitment to the membrane-proximal PKC binding site and subsequent inhibition of PKC activation against the activating stimuli. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 3726~3735 | - |
dc.relation.isPartOf | JOURNAL OF IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | Apoptosis/drug effects | - |
dc.subject.MESH | Apoptosis/genetics | - |
dc.subject.MESH | Apoptosis/immunology* | - |
dc.subject.MESH | Cytoplasm/chemistry | - |
dc.subject.MESH | Cytoplasm/enzymology | - |
dc.subject.MESH | Cytoplasm/genetics | - |
dc.subject.MESH | Cytoplasm/immunology | - |
dc.subject.MESH | Diglycerides/pharmacology | - |
dc.subject.MESH | Fas Ligand Protein | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Ionomycin/pharmacology | - |
dc.subject.MESH | Isoenzymes/antagonists & inhibitors | - |
dc.subject.MESH | Isoenzymes/metabolism | - |
dc.subject.MESH | Jurkat Cells | - |
dc.subject.MESH | Killer Cells, Natural/immunology | - |
dc.subject.MESH | Killer Cells, Natural/metabolism | - |
dc.subject.MESH | Ligands | - |
dc.subject.MESH | Lymphocyte Activation*/drug effects | - |
dc.subject.MESH | Lymphocyte Activation*/genetics | - |
dc.subject.MESH | Membrane Glycoproteins/antagonists & inhibitors | - |
dc.subject.MESH | Membrane Glycoproteins/biosynthesis | - |
dc.subject.MESH | Membrane Proteins/genetics | - |
dc.subject.MESH | Membrane Proteins/immunology | - |
dc.subject.MESH | Membrane Proteins/pharmacology | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Peptide Fragments/genetics | - |
dc.subject.MESH | Peptide Fragments/immunology | - |
dc.subject.MESH | Peptide Fragments/pharmacology | - |
dc.subject.MESH | Phosphatidylserines/pharmacology | - |
dc.subject.MESH | Phytohemagglutinins/pharmacology | - |
dc.subject.MESH | Protein Kinase C/antagonists & inhibitors | - |
dc.subject.MESH | Protein Kinase C/metabolism | - |
dc.subject.MESH | Protein Kinase C-alpha | - |
dc.subject.MESH | Protein Kinase C-theta | - |
dc.subject.MESH | Receptors, Immunologic/biosynthesis | - |
dc.subject.MESH | Receptors, Immunologic/genetics | - |
dc.subject.MESH | Receptors, Immunologic/physiology* | - |
dc.subject.MESH | Receptors, KIR | - |
dc.subject.MESH | Receptors, KIR3DL1 | - |
dc.subject.MESH | Recombinant Proteins/pharmacology | - |
dc.subject.MESH | T-Lymphocytes/cytology | - |
dc.subject.MESH | T-Lymphocytes/drug effects | - |
dc.subject.MESH | T-Lymphocytes/immunology* | - |
dc.subject.MESH | T-Lymphocytes/metabolism* | - |
dc.subject.MESH | Tetradecanoylphorbol Acetate/pharmacology | - |
dc.subject.MESH | Transfection | - |
dc.subject.MESH | fas Receptor/metabolism | - |
dc.title | Molecular mechanism of the activation-induced cell death inhibition mediated p70 inhibitory killer cell Ig-like receptor in Jurkat T cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | Yong Joon Chwae | - |
dc.contributor.googleauthor | Mi Jung Chang | - |
dc.contributor.googleauthor | Sang Myun Park | - |
dc.contributor.googleauthor | Ho Yoon | - |
dc.contributor.googleauthor | Hyun Joo Park | - |
dc.contributor.googleauthor | Se Jong Kim | - |
dc.contributor.googleauthor | Jongsun Kim | - |
dc.identifier.doi | 10.4049/jimmunol.169.7.3726 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00603 | - |
dc.contributor.localId | A00921 | - |
dc.relation.journalcode | J01450 | - |
dc.identifier.eissn | 1550-6606 | - |
dc.identifier.pmid | 12244166 | - |
dc.contributor.alternativeName | Kim, Se Jong | - |
dc.contributor.alternativeName | Kim, Jong Sun | - |
dc.contributor.affiliatedAuthor | Kim, Se Jong | - |
dc.contributor.affiliatedAuthor | Kim, Jong Sun | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 169 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 3726 | - |
dc.citation.endPage | 3735 | - |
dc.identifier.bibliographicCitation | JOURNAL OF IMMUNOLOGY, Vol.169(7) : 3726-3735, 2002 | - |
dc.identifier.rimsid | 49754 | - |
dc.type.rims | ART | - |
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