Complete Nucleotide Sequence of KCNE1 in Korean Genome

Abstract

We have cloned the gene for long QT syndrome in Korean genome and determined its detailed nucleotide sequence. Blood DNAs were isolated from 68 healthy individuals (including males and females) and the genomic DNAs were amplified by PCR method followed by automatic DNA sequencing. Entire sequence of the coding region for KCNE1 was located in exon 3. PCR products were reexamined for the confirmation of KCNE1-specific amplification by nested PCR KCNE1 mRNA was 436 bp. This corresponded to 129 amino acids. There was no recognizable difference between males and females. This study should contribute to the better understanding of long QT syndrome in Korean population.