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Mycophenolic acid가 배양된 사람혈관평활근세포의 증식과 그에 관여하는 세포내 신호전달계에 미치는 영향

Other Titles
 Effect of Mycophenolic Acid on Human Vascular Smooth Muscle Cell Proliferation and Its Signal Transduction 
Authors
 박제현  ;  하헌주  ;  김명수  ;  서지연  ;  김혜진  ;  박기일  ;  김유선 
Citation
 JOURNAL OF THE KOREAN SURGICAL SOCIETY , Vol.62(1) : 1-7, 2002 
Journal Title
 JOURNAL OF THE KOREAN SURGICAL SOCIETY 
ISSN
 1226-0053 
Issue Date
2002
Keywords
Mycophenolic acid ; Human vascular smooth muscle cell ; Reactive oxygen species ; Mitogenactivated protein kinase
Abstract
Purpose : Vascular smooth muscle cells (VSMCs) migration and proliferation play important roles in chronic allograft rejection. Mycophenolic acid (MPA) inhibits the proliferation of VSMCs, glomerular mesangial cells and fibroblasts as well as lymphocytes. Since reactive oxygen species (ROS) and mitogen-activated protein kinase (MAPK) play important roles in the proliferation of VSMCs, the present study examined the effects of MPA on intracellular ROS generation, activation of ERK and p38 MAPK, and the proliferation of VSMCs cultured under platelet derived growth factor (PDGF). Methods : Human VSMCs obtained from ATCC were cultured with RPMI-1640 containing 10% fetal bovine serum. Near confluent VSMCs were incubated with serum-free media for 48 hours to arrest and synchronize the cell growth. MPA was administered 1hour before the addition of PDGF. 5-(and-6)-chloromethyl-2’,7’-dichlorodihydrofluorescein (DCF)-sensitive intracellular ROS was detected by FACS. Activations of ERK1/ERK2 and p38 MAPK were measured by Western blot analysis. Proliferation of VSMC was assessed by [3H]-thymidine incorporation. Results PDGF administered at 10 ng/ml, which induced human VSMCs proliferation, rapidly increased intracellular ROS by 1.6-fold (P<0.05), ERK1/ERK2 activation by 2.1-fold, (P<0.05) and p38 MAPK activation by 1.9-fold (P<0.05), respectively, as compared to the control. MPA 1 and 10μM effectively inhibited PDGF-induced human VSMCs proliferation. MPA also effectively inhibited PDGF-induced intracellular ROS generation as well as ERK1/ERK2 and p38 MAPK activation. Conclusion : The present study suggests that MPA inhibits PDGF-induced human VSMCs proliferation, possibly by inhibiting intracellular ROS generation and the phosphorylation of ERK1/ERK2 and p38 MAPK.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Yu Seun(김유선) ORCID logo https://orcid.org/0000-0002-5105-1567
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/144367
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