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Macrophage와 Glycated-LDL의 Uptake 및 산화에 미치는 몇 가지 Effector의 효과

Other Titles
 Effects of Effectors on Uptake and Oxidation of Glycated-LDL 
Authors
 조현미  ;  유승재  ;  정희경  ;  황기철  ;  장양수  ;  신승언  ;  김태웅 
Citation
 한국지질동맥경화학회지, Vol.12(2) : 430-441, 2002 
Journal Title
 한국지질동맥경화학회지 
ISSN
 1226-4393 
Issue Date
2002
Keywords
glyco-oxidation ; glycated-LDL ; uptake test ; macrophage ; LPS (Lipopolysaccharide) ; M-CSF (macrophage-Colony Stimulating Factor) ; PMA (Phorbol-12 Myristate13-Acetate) ; dexamethason
Abstract
Objective: Diabetes is associated with accelerated atherosclerosis, with the incidence of cardiovascular disease in diabetic patients, three to four times that of nondiabetic individuals. Glucose reacts with LDL in a process known as nonenzymatic glycation. Also, oxidation of LDL is a factor in the develomemt of atheroclerosis. From the previous study, we found that M-CSF and PMA increased the viability of macrophage while LPS and dexamethasone decreased. In the present study, effects of effectors on uptake and oxidation of glycated-LDL on HMDM(human monocyte derived macrophage) were tested. Methods: Glycated-LDL was labelled with 5-iodoacetamide(5-IAF) in order to investigate both binding and degradation of glycated-LDL by human monocyte derived macrophage. Also, the electrophoretic mobility and thiobarbituric acid reactive substance(TBARS) value of glycated-LDL were compared. Results: From the experimental results on the effects of target molecules on glyco-oxidation of LDL, M-CSF and PMA enhanced the LDL glycoxidation and mobilities on agarose gel electrophoresis whereas LPS and dexamethasone decreased. Also, effects of effectors on the viability of macrophage and uptake of glycated-LDL showed the same results from the glycoxidation of LDL. Conclusion: Finally, we concluded that effects of effectors on the viability of macrophage and uptake of glycated-LDL showed the same pattern compared to the glycoxidation of LDL. And it appears that factors such as viability, macrophage uptake, and glycoxidation are related on the development of atherosclerosis.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Jang, Yang Soo(장양수) ORCID logo https://orcid.org/0000-0002-2169-3112
Hwang, Ki Chul(황기철)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/144350
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