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Macrophage 분화에 미치는 Glycated-LDL 및 몇 가지 Effector의 영향

Other Titles
 Cell differentiation of macrophage with glycated-LDL in the presence of effectors 
 조현미  ;  유승재  ;  정희경  ;  오수영  ;  황기철  ;  장양수  ;  신승언  ;  김태웅 
 한국지질동맥경화학회지, Vol.12(2) : 442-452, 2002 
Journal Title
Issue Date
Glycated-LDL (gly-LDL) ; Atherosclerosis ; macrophage ; M-CSF (Macrophage-Colony Stimulating Factor) ; PMA (Phorbol-12 Myristate 13-Acetate) ; LPS (Lipopolysaccharide) ; Dexamethason
Objective: Glycation of LDL is significantly increased in diabetic patients. There is accumulating evidence that glycation of circulating lipoproteins, is implicated in the pathogenesis of diabetic atherosclerosis. Glycation, the nonenzymatic binding of glucose to protein molecules, can increase the atherogenic potential of certain plasma constituents, including low-density lipoprotein (LDL). In the present experiment, we investigated the proliferation of macrophage with glycated-LDL in the presence of several effectors such as M-CSF (Macrophage-Colony Stimulating Factor), PMA (Phorbol-12 Myristate 13-Acetate), LPS (Lipopolysaccharide) and dexamethasone. Methods: Fresh LDL was glycated for 0, 10, 30, 60 and 180 days and differentiation of human monocyte derived macrophage(HMDM) was tested. Also, glycated-LDL (50㎍/mL) with effectors such as M-CSF(15 ng/mL), PMA(120 ng/mL), LPS(10ng/ml) and dexamethason(1㎍/ml) were incubated on HMDM and compared differentiation with MTT assay. Results: From the results, glycation of LDL increased differentiation of macrophage. And, M-CSF and PMA increased the differentiation of macrophage whereas LPS and dexamethason decreased. Conclusion: It is thought that several effectors are involved in development of atherosclerosis, and we obtained the following conclusion that M-CSF and PMA might influence the development of atheroclerosis because they increased the viability of macrophage.
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1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Hwang, Ki Chul(황기철)
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