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In vivo differentiation of mouse embryonic stem cells into hepatocytes

Authors
 D. Choi  ;  H.J. Oh  ;  U.J. Chang  ;  S.K. Koo  ;  J.X. Jiang  ;  S.Y. Hwang  ;  J.D. Lee  ;  G.C. Yeoh  ;  H.S. Shin  ;  J.S. Lee  ;  B. Oh 
Citation
 CELL TRANSPLANTATION, Vol.11(4) : 359-368, 2002 
Journal Title
 CELL TRANSPLANTATION 
ISSN
 0963-6897 
Issue Date
2002
MeSH
Animals ; Cell Differentiation ; Cell Lineage ; Coloring Agents/pharmacology ; Embryo, Mammalian/cytology* ; Hepatocytes/cytology* ; Hepatocytes/metabolism ; Immunohistochemistry ; Liver/cytology ; Liver/metabolism ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Periodic Acid/pharmacology ; Phenylalanine Hydroxylase/metabolism ; RNA, Messenger/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Stem Cells/cytology* ; Teratoma/metabolism ; Transaminases/metabolism
Abstract
Embryonic stem (ES) cells have been regarded as a powerful resource for cell replacement therapy. In recent reports mouse ES cells have been successfully applied in the treatment of spinal cord injury, hereditary myelin disorder of the central nervous system, and diabetes mellitus. Another type of disease that could benefit from the availability of stem cell therapy is liver disease. However, for this potential to be realized, it is necessary to demonstrate the differentiation of ES cells into hepatocytes. To demonstrate the in vivo differentiation potential of mouse ES cells, we injected ES cells into the spleen of immunosuppressed nude mice. Histological analysis of teratomas derived from injected ES cells revealed that some areas contained typical hepatocytes arranged in a sinusoidal structure. The hepatic nature of these cells was further confirmed by showing that transcripts of liver-specific genes were present in the differentiated teratoma using reverse transcriptase-polymerase chain reaction and immunohistochemistry using several liver-specific antibodies including HEP-PAR, phenylalanine hydroxylase, and mouse N-system aminotransferase to identify the respective proteins in the differentiated hepatocytes. This is the first demonstration that mouse ES cells can differentiate in vivo into a mixed population of hepatocytes of varying maturity. This finding extends the potential use of ES cells in the cell replacement therapy by including its possible application for treating liver diseases.
Full Text
http://www.ingentaconnect.com/content/cog/ct/2002/00000011/00000004/ct285?token=00561da73136e0b1407ae573d257025704423565f7a40344d7c3a677c4e75477e4324576b642738fe138b2
DOI
10.3727/000000002783985792
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Jin-Sung(이진성) ORCID logo https://orcid.org/0000-0002-1262-8597
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/144212
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