Cited 157 times in
Cathepsin D produces antimicrobial peptide parasinⅠfrom histone H2A in the skin mucosa of fish.
DC Field | Value | Language |
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dc.contributor.author | 이원택 | - |
dc.date.accessioned | 2016-05-16T11:08:30Z | - |
dc.date.available | 2016-05-16T11:08:30Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143880 | - |
dc.description.abstract | Parasin I is a potent 19-residue antimicrobial peptide isolated from the skin mucus of wounded catfish (Parasilurus asotus). Here we describe the mechanism of parasin I production from histone H2A in catfish skin mucosa on epidermal injury. Cathepsin D is found to exist in the mucus as an inactive proenzyme (procathepsin D), and a metalloprotease, induced on injury, cleaves procathepsin D to generate active cathepsin D. This activated form of cathepsin D then cleaves the Ser19-Arg20 bond of histone H2A to produce parasin I. Immunohistochemical analysis reveals that unacetylated histone H2A, a precursor of parasin I, and procathepsin D are present in the cytoplasm of epithelial mucous cells and that parasin I is produced on the mucosal surface on epidermal injury. Western blot analysis shows that parasin I is also present in the skin mucus of other fish species. Furthermore, parasin I shows good antimicrobial activity against fish-specific bacterial pathogens. Taken together, these results indicate that cathepsin D and a metalloprotease participate in the production of parasin I from histone H2A and that parasin I contributes to the innate host defense of the fish against invading microorganisms. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 429~431 | - |
dc.relation.isPartOf | FASEB JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Anti-Infective Agents/chemistry | - |
dc.subject.MESH | Anti-Infective Agents/metabolism* | - |
dc.subject.MESH | Anti-Infective Agents/pharmacology | - |
dc.subject.MESH | Antimicrobial Cationic Peptides/biosynthesis* | - |
dc.subject.MESH | Antimicrobial Cationic Peptides/chemistry | - |
dc.subject.MESH | Antimicrobial Cationic Peptides/pharmacology | - |
dc.subject.MESH | Catfishes/anatomy & histology | - |
dc.subject.MESH | Catfishes/metabolism* | - |
dc.subject.MESH | Cathepsin D/metabolism | - |
dc.subject.MESH | Cathepsin D/physiology* | - |
dc.subject.MESH | Enzyme Precursors/metabolism | - |
dc.subject.MESH | Epidermis/metabolism | - |
dc.subject.MESH | Fish Diseases/metabolism | - |
dc.subject.MESH | Fish Diseases/pathology | - |
dc.subject.MESH | Histones/metabolism* | - |
dc.subject.MESH | Metalloendopeptidases/metabolism | - |
dc.subject.MESH | Microbial Sensitivity Tests | - |
dc.subject.MESH | Models, Biological | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Mucous Membrane/metabolism | - |
dc.subject.MESH | Mucous Membrane/pathology | - |
dc.subject.MESH | Protein Precursors/metabolism | - |
dc.subject.MESH | Sequence Alignment | - |
dc.subject.MESH | Skin/metabolism* | - |
dc.subject.MESH | Skin/pathology | - |
dc.subject.MESH | Skin Diseases/metabolism | - |
dc.subject.MESH | Skin Diseases/pathology | - |
dc.title | Cathepsin D produces antimicrobial peptide parasinⅠfrom histone H2A in the skin mucosa of fish. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Anatomy (해부학) | - |
dc.contributor.googleauthor | Ju Hyun Cho | - |
dc.contributor.googleauthor | In Yup Park | - |
dc.contributor.googleauthor | Hun Sik Kim | - |
dc.contributor.googleauthor | Won Taek Lee | - |
dc.contributor.googleauthor | Mi Sun Kim | - |
dc.contributor.googleauthor | Sun Chang Kim | - |
dc.identifier.doi | 10.1096/fj.01-0736fje | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03007 | - |
dc.relation.journalcode | J00889 | - |
dc.identifier.eissn | 1530-6860 | - |
dc.identifier.pmid | 11821259 | - |
dc.contributor.alternativeName | Lee, Won Taek | - |
dc.contributor.affiliatedAuthor | Lee, Won Taek | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 16 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 429 | - |
dc.citation.endPage | 431 | - |
dc.identifier.bibliographicCitation | FASEB JOURNAL, Vol.16(3) : 429-431, 2002 | - |
dc.identifier.rimsid | 37630 | - |
dc.type.rims | ART | - |
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